PLASMID PROFILING AND CURING OF METHICILLIN-RESISTANT STAPHYLOCOCCUS EPIDERMIDIS (MRSE) AND METHICILLINSENSITIVE STAPHYLOCOCCUS EPIDERMIDIS (MSSE) ISOLATES
Journal Title: WORLD JOURNAL PHARMACY AND PHARMACEUTICAL SCIENCE - Year 2017, Vol 6, Issue 3
Abstract
The minimum inhibitory concentration of antibiotics (penicillin and tetracycline) to two methicillin resistance S. epidermidis isolates had determined, they were 60U and 25μg/ml respectively for the isolate 32S, they were 40U of penicillin for the isolate 40S.Results from agarose gel electrophoresis showed two isolates of MSSE 5S and 51S don't have any plasmid while other two isolates of MRSE have plasmids, two plasmids found in S. epidermidis isolate (40S), one of them was large plasmid and the other was small plasmid and in S. epidermidis isolate (32S) contained three plasmids, one from these was large plasmid and the other two plasmids were small plasmids. The results of plasmid curing by using sodium dodecyl sulphate (SDS) and rifampin showed that the plasmids of the local isolates 32S and 40S was curred by treatment with 100μg/ml, 230μg/ml respectively from SDS and 150μg/ml, 200μg/ml respectively from rifampin.Cured cells from 40S isolate (MRSE) were sensitive to penicillin and still resistance to methicillin and the cured cells from 32S isolate were sensitive to penicillin, tetracycline and still resistance to methicillin.
Authors and Affiliations
Dr. May T. Flayyih
Keywords
Related Articles
- EP ID EP617773
- DOI -
- Views 157
- Downloads 0
THE IMPACT OF THE SYNTHETIC FLUOXETINE AND HERBAL ST.JHON’S WORT ON THE FETUS BRAIN OF DEPRESSED MOTHERS TREATED WITH RESERPINE
Depression has been known as a major public health problem. Women are at greatest risk of suffering from depression during the childbearing years and those may either become pregnant while taking an antidepressant or may...
REVIEW ON RUTA GRAVEOLENS
Bioassay-directed isolation of antifungal compounds from an ethyl acetate extract of Ruta graveolens leaves yielded two furanocoumarins, one quinoline alkaloid, and four quinolone alkaloids including a novel compound, 1-...
ROLE OF EPIDURAL STEROID INJECTION IN MANAGEMENT OF HERNIATED INTERVERTEBRAL DISC WITH RADICULOPATHY
Backgroung: Epidural steroid injection is an important modality in the conservative management of herniated intervertebral disc with radiculopathy. Epidural steroid injections (ESI) have been used since the 1950s for tr...
ANTIDIABETIC ACTIVITY OF BALANITES AEGYPTIACA (L.) DELILE LEAVES IN EXPERIMENTAL DIABETIC RATS
Screening of antidiabetic activity of methanolic extract of Balanites aegyptiaca (L.) Delile (leaves) was done in rats by conducting glucose tolerance test (GTT) study and evaluating their effects (single dose and multid...
SURGICAL MANAGEMENT OF PAGET’S DISEASE OF THE VULVA: A CASE REPORT
Extra-mammary Paget disease (EMPD) is one of the rare neoplastic conditions of the skin that accounts for less than 1% of the vulvar malignancies. The condition typically presents as a red, velvety, pruritic skin rash of...