Plastic (LDPE) Degradation by Induced Mutations In Pseudomonas Putida
Journal Title: IOSR Journal of Environmental Science, Toxicology and Food Technology (IOSR-JESTFT) - Year 2018, Vol 12, Issue 9
Abstract
Polythene is considered as one of the important object used in daily life. Being versatile in nature and resistant to microbial attack, they effectively cause environmental pollution. The present investigation has focused the aspect through microbial assisted biodegradation of low-density polyethylene (LDPE) have been performed using Pseudomonas putida. The global utility of polyethylene is escalating at a velocity of 12% per annum. In recent times, the biodegradation of plastic waste and the use of microorganisms to degrade the polymers have gained remarkable magnitude because of the inefficiency of the chemical and physical disposal methods, and the ecological harms they cause. Environmental degradation of PE (polyethylene) proceeds by synergistic action of photo- and thermo-oxidative degradation and biological activity (i.e., microorganisms); in natural form it is not biodegradable. The making of the genetically engineered microbes for bioremediation, the latter being a strategy to develop an accelerated evolution of pathways by DNA restructuring the treatment of LDPE film with mutated Pseudomonas putida (MTCC NO: 2467) showed reduced tensile strength in 15 days were 0.22 when compared to 60 days 0.15. Media pH and viability and growth, concentration matters for ideal polymer degradation, in positive control it is 6.8, when compared to induced mutation strain is 8.5. When LDPE films were exposed to mutated Pseudomonas putida (60min), degradation rate based on weight loss, were noticed 34% against positive control 11% for 60 days, followed by DNA isolation for Gel electrophoresis, and Mutated DNA Stability analysis by Capillary Gel electrophoresis were carried out
Authors and Affiliations
Anantharam. H1* ,, Muralidhar. S. Talkad2
Keywords
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