Platelet Adhesion Comparison of Silk Fibroin Dissolved by Different Solvents
Journal Title: Biomedical Journal of Scientific & Technical Research (BJSTR) - Year 2019, Vol 12, Issue 5
Abstract
Silk fiber is a natural protein with high purity, and can be easily processed into films, mesh and porous materials, which possesses good cytocompatibility and biodegradability, especially excellent water absorption and breathable. Base on the satisfactory properties, we prepared regenerated silk fibroin materials using neutral salt solvents dissolution with CaCl2, Ca(NO3)2 and LiBr, respectively (named SF-CaCl2, SF-LiBr and SF-Ca(NO3)2, and investigated platelet adhesion ability to evaluate driving force of hemostasis. Results showed that platelet adhesion rate and platelet activity on the calcium salt dissolved silk fibroin film surfaces were significantly higher that on the SF-LiBr film. SEM images showed that platelets tended to aggregate on the SF-CaCl2 and SF-Ca(NO3)2 films.Silk has rich sources and high purity, is composed of silk fiber core layer (silk fibroin) and sericin wrapped outer layer. Silk fibroin can be easily processed into various morphological materials, such as nanofibers, films, sponges, gels or nanoparticles [1-5], and has been widely studied for applications in extracellular matrix, tissue engineering scaffolds and drug delivery carriers, owing to its excellent biocompatibility and biodegradability [1-11]. On the other hand, silk fibroin contains more hydrophilic amino acids, such as serine, Tyrosine, glutamic acid, aspartic acid and lysine [12-14], endowing silk fibroin fast water absorption. On the bleeding wounds, rapid water absorption of a material is beneficial for improving blood viscosity, and promoting platelet activation and aggregation, thus accelerating clotting. Otherwise, the functional groups -COOH from glutamic acid or aspartic acid, and -OH from serine or Tyrosine can interact with Ca2+ by coordination bonds and electrostatic interaction [15,16]. In the coagulation mechanism, it is well known that Ca2+ is a key clotting factors, which interacts with platelet surface phospholipids to activate thrombin, and then digest fibrinogen into fibrin, resulting in coagulation formation. Therefore, silk fibroin protein is worth considering for the development of hemostatic materials. Platelets are the drivers in cascade pathway for blood clotting. In the present paper, we explored platelet adhesion and aggregation activity on regenerated silk fibroin materials prepared by different dissolution mechanisms, expecting to provide valuable guidance for the development of silk fibroin blood contact materials.
Authors and Affiliations
Helei Li, Wei Tian, Guangzhou Song, Mengyao Ding, Honggen Yi, Yin Yin, Fenglin Dong, Jiannan Wang
Keywords
Related Articles
- EP ID EP587489
- DOI 10.26717/BJSTR.2019.12.002310
- Views 198
- Downloads 0
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