Polygalacturonase production by Aspergillus nomius MR103 in solid state fermentation using Agro-industrial wastes
Journal Title: Journal of Applied and Natural Science - Year 2019, Vol 11, Issue 2
Abstract
The present study was aimed at polygalacturonase production from Aspergillus nomius MR103 under solid state fermentation. A total of 57 fungal strains were obtained from mangrove soils collected from Gilakaladindi and Malakayalanka of Krishna District Andhra Pradesh. For the isolation of fungi these Soil samples were serially diluted and plated on pectin agar media plates. Among them, the isolate which showed maximum polygalacturonase activity was selected for this study. This strain was identified as A. nomius MR 103 by 18S rRNA sequences analysis. Pectin rich agro-industrial wastes like apple peel, citrus peel, orange peel, wheat bran, rice bran and sugarcane bagasse were used as substrates for polygalacturonase production by A. nomius MR 103. This strain was inoculated into the nutrient broth containing agro industrial wastes under solid state fermentation and amount of Polygalacturonase production was estimated. Maximum enzyme production of 4.83 IU/mg was recorded at pH 7.0 and temperature 35?C after 7 days of incubation, when orange peels were used as substrate. Addition of carbon and nitrogen sources to orange peel media improved the Polygalcturonase production. Sucrose as carbon and peptone as nitrogen sources were proved to be the best for maximum production of Polygalcturonase by A. nomius MR 103 on orange peel substrate. Utilization of agro-industrial by-products provided the establishment of a cost-efficient and sustainable process for enzyme production.
Authors and Affiliations
Ravi Ketipally, G. Kranthi Kumar, M. Raghu Ram
Keywords
Related Articles
- EP ID EP588661
- DOI 10.31018/jans.v11i2.2039
- Views 101
- Downloads 0
Ferti-irrigational impact of distillery effluent and Di-ammonium phosphate on the soil and growth characteristics of Egg plant (Solanum melongena L.)
A comparative study was conducted to assess the ferti-irrigational effect of Distillery effluent (DE) concentrations such as 10%, 25%, 50%, 75% and 100% along with control (Bore well water-BWW) and Di-ammonium phosphate...
Growth promotion of transplanted rice plant by bioagents effective against bacterial leaf blight disease of rice under glasshouse conditions
The experiment was conducted to evaluate growth promotion of transplanted rice by fungal and bacterial bioagents effective against bacterial leaf blight of rice, under glasshouse conditions. Bioagent formulations (PBA -1...
Seasonal abundance and bio-efficacy of different insecticides against castor semilooper on castor
The present study was conducted at Chaudhary Charan Singh Haryana Agricultural University, Hisar, Regional Research Station, Bawal, Haryana (India). The larvae of castor semilooper remain active from 30th standard weeks...
Genetic variability for grain yield and water use efficiency in blackgram genotypes
Transpiration efficiency (TE, g biomass kg-1 water transpired) is the preferred measure for examining po- tential genetic variation in crop water use efficiency (WUE). TE was assessed gravimetrically from sowing to grain...
Process optimization of supercritical carbon dioxide (SC-CO2) extraction parameters for extraction of deoxynojirimycin (1-DNJ) from mulberry (Morus alba L.) leaves
In the present study, supercritical fluid extraction (SFE) technology was applied to extract deoxynojirimycin (1-DNJ) from mulberry leaf powder using carbon dioxide (CO2) as major extraction solvent with ethanol as cosol...