POLYMORPHISM OF CYTOCHROME P450, SUPERFAMILY19, POLYPEPTIDE 1 GENE AND RELATED TO AROMATASE ENZYME ACTIVITY IN ACROMEGALY IRAQI PATIENTS
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2017, Vol 10, Issue 6
Abstract
Objective: The objective of the present work was to investigate the association between aromatase enzyme activity and polymorphisms, rs2236722, in exon 2 of cytochrome P450 (CYP), superfamily19, polypeptide 1 (CYP19A1gene) in patients with active acromegaly.Methods: A total of 120 males and females (age 20-60 years) were enrolled in this study, 60 patients with active acromegaly who have attended the National Diabetes Centre and and Specialist Center for endocrinology and diabetes, Baghdad, from December 2015 to June 2016 and 60 healthy individuals with matches as a control group. From the whole blood, genomic DNA was extracted to perform genotyping analysis and single-nucleotide polymorphism, rs2236722, in the CYP19A1 gene using multiplex polymerase chain reaction technique, and serum aromatase levels were determined using a solid-phase enzyme-linked immunosorbent assay based on sandwich method.Results: The results show that the aromatase activity levels of patients were a highly significant decrease when contrasted with healthy control groups in both sexes (p≤0.01), and there were summarized positive results of an allele in single-nucleotide polymorphism, rs2236722, were highly significant in TT allele when contrasted with healthy control groups. While revealed that there was non-significant difference in TC allele when contrasted with healthy control groups (p>0.01).Conclusion: we conclude that there was associated between CYP19A1 gene polymorphism (rs2236722) with aromatase activity and related to acromegaly patients.
Authors and Affiliations
Iqbal Hanash Dhefer, Salma Abdul Rudha Abbas, Najwa Sh Ahmed
Keywords
Related Articles
- EP ID EP605341
- DOI 10.22159/ajpcr.2017.v10i6.17886
- Views 113
- Downloads 0
SYNTHESIS OF NEW BORON COMPOUNDS WITH AMOXICILLIN AND SOME OF ITS METAL COMPLEXES WITH USE THEM IN ANTIBACTERIAL, ASSESSMENT OF HEPATOPROTICTIVE AND KIDNEYACTIVITY, ANTICANCER AND ANTIOXIDANT APPLICATIONS
Objective: New ligand ({7-[2-amino-2-(4-hydroxyphenyl)-acetamido-3,3-dimethyl-6-oxo-2-thia-5-aza bicyclo[3,2,0]heptane-4-carboxylic boric anhydride}) with its Co (II), Ni (II) and Cu (II) complexes. And new mixed ligand...
PATIENTS’ KNOWLEDGE, ATTITUDES, AND PRACTICES ON UNCOMPLICATED MALARIA MANAGEMENT IN PLATEAU STATE, NORTH-CENTRAL NIGERIA
Objectives: This study aimed to assess patients’ knowledge, attitudes, and practices (KAP) on uncomplicated malaria management in primary health-care (PHC) facilities of Plateau state, Nigeria. Methods: A validated self...
FORMULATION AND OPTIMIZATION OF NANOSUSPENSION FOR IMPROVING SOLUBILITY AND DISSOLUTION OF GEMFIBROZIL
Objective: The study aims at the formulation and optimization of gemfibrozil (Gem) nanosuspension (NS) for improving its solubility and dissolution rate.Method: Gem NS was prepared by precipitation-ultrasonication method...
COCRYSTALS: A COMPLETE REVIEW ON CONVENTIONAL AND NOVEL METHODS OF ITS FORMATION AND ITS EVALUATION
The active moiety with poor solubility is posing a challenge in drug development which may reduce the effectiveness in patients when administered orally. Cocrystal formation is one of the latest approaches for improving...
IN VITRO EVALUATION OF THE ANTHELMINTIC ACTIVITY OF RHIZOME EXTRACTS OF CURCUMA LONGA (LINN.)
Objective: The current study was carried out to evaluate the anthelmintic activity of the rhizome extract of Curcuma longa as an alternative source of effective remedies for nematodiasis.Methods: The anthelmintic activit...