Polymorphisms of Estrogen Receptor-α and Estrogen Receptor-β Genes and its Expression in Endometriosis
Journal Title: Turkish Journal of Pharmaceutical Sciences - Year 2021, Vol 18, Issue 1
Abstract
Objectives: Endometriosis is a common gynecological disorder, characterized by the presence of endometrial-like tissue in the extrauterine location. The increasing estradiol concentration can influence endometriosis risk and estrogen receptor (ER) activity. Polymorphism in ER causes gene expression alteration and influences hormone-receptor interaction. This research aims to determine ER genetic polymorphisms in endometriosis pathogenesis. Materials and Methods: This study was performed on case-control polymorphisms, which compared 83 women with endometriosis and 76 women without endometriosis. However, the samples used for ER gene expression analysis and estrogen level measurement were obtained from 18 women with endometriosis and 18 women without endometriosis. Polymerase chain reaction-restriction fragment length polymorphism was used to determine ER genetic polymorphisms. Chi-square, Mann-Whitney test, Spearman’s correlation (p), t-independent, and two-tailed tests were used to analyze the data. Results: Association between the allele ERα rs9340799 A/G and endometriosis was significantly different (p=0.012), whereas rs2234693 T/C polymorphism showed no association with endometriosis. The correlation between the genotype frequencies of allele ERβ rs4986938 G/A and endometriosis was found significantly different (p=0.015; p=0.034). Conclusion: Estradiol level and ERβ expression increases, polymorphism genotypes and alleles of ERβ rs4986938 G/A gene and allele frequency of ERα rs9340799 A/G gene have roles in endometriosis.
Authors and Affiliations
Eldafira ELDAFIRA, Vivitri Dewi PRASASTY, Abinawanto ABINAWANTO, Luthfiralda SYAHFIRDI, Dwi Ari PUJIANTO
Keywords
Related Articles
- EP ID EP693625
- DOI 10.4274/tjps.galenos.2019.94914
- Views 104
- Downloads 0
Microemulsion Based Gel of Sulconazole Nitrate for Topical Application
Objectives: Sulconazole is a broad spectrum antifungal agent of the imidazole class used against dermatophytes and other fungi to treat skin infections. The aim of the present work was to formulate and evaluate a microem...
Development and Validation of a Stability Indicating RP-HPLC Method for Simultaneous Estimation of Teneligliptin and Metformin
Objectives: The main objective of the present work is to develop a simple, precise, specific and stability method indicating reverse phase high performance liquid chromatography method for simultaneous estimation of tene...
In Situ Absorption Study of Acebutolol by Modulating P-glycoprotein with Verapamil in Rats
Objectives: Acebutolol HCl (ABL) is a selective β-adrenergic receptor blocking agent that is preferably administered by the oral route despite its low bioavailability (30-50%). The purpose of this study was to evaluate t...
Determination of Potential Drug–Drug Interactions Using Various Software Programs in a Community Pharmacy Setting
Objectives: The aim of the present study was to compare various software programs in detecting potential drug–drug interactions in a community pharmacy setting. Materials and Methods: Details of prescriptions were colle...
Decreased Protein Kinase C Expression in the Cochlear Fibroblasts of Diabetic Rat Models Induced by Curcumin
Objectives: Microcirculation and hemodynamic disturbances, including in the cochlea, are commonly found in diabetic patients. A study on diabetic rats discovered histopathological changes in outer hair cells and the spir...