Population Structure and Genetic Diversity of the Medicinal and Aromatic Plant Cunila spicata Benth. (Lamiaceae) Based on Inter-Simple Sequence Repeat (ISSR) Markers
Journal Title: JOURNAL OF ADVANCES IN BIOTECHNOLOGY - Year 2015, Vol 5, Issue 2
Abstract
Cunila spicata is an endangered aromatic and medicinal plant of South Brazil. In the present paper, the ISSR technique was employed to study the intra- and inter-population genetic diversity of this species. Nine primers generated a total of 109 amplification products, most of which were polymorphics. Low genetic diversity at population level (HE= 0.053) and species level (HT=0.196), and high differentiation among populations (GST= 0.727) were detected in C. spicata. The genetic diversity, low estimated genetic flow and absence of correlation between genetic distances, geographic distances and chemical composition, indicates that genetic drift and inbreeding may be the main factors involved in the genetic structure of C. spicata. Based on these findings, strategies are proposed for the genetic conservation and management of this species.
Authors and Affiliations
Ana Paula Longaray Delamare, Sergio Echeverrigaray, Marcos Albuquerque, Jucimar Zacaria
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