Potential application of CuSO4 for the reduction of lipofuscin autofluorescence in formalin-fixed paraffin-embedded tissue
Journal Title: Bioresearch Communications - Year 2016, Vol 2, Issue 2
Abstract
The fluorescent pigment lipofuscin accumulates within the cytoplasm of cells of most of the organs. Because of its broad excitation and emission spectra, the presence of lipofuscin autofluorescence complicates the use of fluorescence microscopy. In this study, we examined chemical treatments of tissue sections with two concentration of CuSO4 for their ability to reduce or eliminate lipofuscin-like autofluorescence without adversely affecting other fluorescent labels. We found that 4mM CuSO4 in 50 mM ammonium acetate buffer mildly eliminated lipofuscin autofluorescence in sections of human tonsil or mouse synovial tissue, whereas, at higher concentration (10mM) the autofluorescence was almost completely abolished. We found that this treatment had no effect on the fluorescence intensity of target tissue. We conclude that treatment of tissue with CuSO4 provides a reasonable compromise between reduction of lipofuscin-like fluorescence and maintenance of specific fluorescent labels.
Potential application of CuSO4 for the reduction of lipofuscin autofluorescence in formalin-fixed paraffin-embedded tissue
The fluorescent pigment lipofuscin accumulates within the cytoplasm of cells of most of the organs. Because of its broad excitation and emission spectra, the presence of lipofuscin autofluorescence complicates the use of...
Antioxidants and Total Phenolics of Ludwigia repens rubin Partially Improve the Hepatic Damage Induced by Carbon Tetra Chloride in Animal Model
This research investigated the antioxidative and hepatoprotective properties of Ludwigia repens methanol extract. Antioxidative effects were measured by DPPH radical scavenging method and FeCl3 reducing power assay metho...
Physicochemical and cooking properties of short grain aromatic Kalijira rice cultivars in Bangladesh
In the present study we have selected a total of 55 small grain aromatic rice varieties including 49 accessions of Kalijira and 6 popular and local high yielding variety (HYV) seeds were collected from GRS Division, Bang...
Drug Resistance, Serological Study and Plasmid Profile Analysis of Bacterial Isolates from Anorectal Sepsis
Anorectal sepsis is a clinical state accompanied by the infection of the anorectal region of the human body and inappropriately treated sepsis may result in increased morbidity and mortality. Common anorectal sepsis case...
Betulin-3-caffeate and Amyrin from the Stem Bark of Barringtonia acutangula (L
Barringtonia acutangula (Indian Oak) is an important medicinal plant of Bangladesh and India for its phytochemical and prognostic biomarkers in stem. This plant was selected based on its medicinal properties including an...