Potential application of CuSO4 for the reduction of lipofuscin autofluorescence in formalin-fixed paraffin-embedded tissue
Journal Title: Bioresearch Communications - Year 2016, Vol 2, Issue 2
Abstract
The fluorescent pigment lipofuscin accumulates within the cytoplasm of cells of most of the organs. Because of its broad excitation and emission spectra, the presence of lipofuscin autofluorescence complicates the use of fluorescence microscopy. In this study, we examined chemical treatments of tissue sections with two concentration of CuSO4 for their ability to reduce or eliminate lipofuscin-like autofluorescence without adversely affecting other fluorescent labels. We found that 4mM CuSO4 in 50 mM ammonium acetate buffer mildly eliminated lipofuscin autofluorescence in sections of human tonsil or mouse synovial tissue, whereas, at higher concentration (10mM) the autofluorescence was almost completely abolished. We found that this treatment had no effect on the fluorescence intensity of target tissue. We conclude that treatment of tissue with CuSO4 provides a reasonable compromise between reduction of lipofuscin-like fluorescence and maintenance of specific fluorescent labels.
A Cursory Look at the Pharmacy Practice and Pharmaceutical Care in the Public Hospital(s) of Bangladesh: A Case Study
Pharmaceutical care of a country depends on overall Health system of that particular country. This sector of Bangladesh is one of the much neglected one. Objective of the study is to ascertain the status of the Pharmaceu...
Comparative Analysis of the Microbiological Quality between Industrially Produced and Street Vended Ice Creams Offered for Public Consumption in Dhaka City, Banglades
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Isolation of Bioactive Principles and Studies of Antimicrobial, Cytotoxic and Antioxidant Activities of the stem bark of Baccaurea ramiflora (Euphorbiaceae
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In vitro Efficacy of Plant Extracts on Seed Germination and Fungal Infection of Six Varieties of Wheat (Triticum aestivum L.)
The fungitoxicity of six plants extract viz., Allium sativum L., Azadirachta indica A. Juss., Citrus limon (L.) Burm. f., Datura metel L., Gynura procumbens Merr. and Thuja occidentalis L. were evaluated on seed germinat...
Identification and expression profiling of microRNAs and their corresponding targets related to phytoremediation of heavy metals in jute (Corchorus olitorius var. O-9897)
A cost effective plant-based approach for the remediation of contaminated soil and water that pose a major environmental and human health problem, is termed phytoremediation. It takes advantage of the remarkable ability...