POTENTIAL ROLE OF MILK THISTLE SEED AND ITS OIL EXTRACTS AGAINST HEART AND BRAIN INJURIES INDUCED BY γ-RADIATION EXPOSURE
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2017, Vol 9, Issue 7
Abstract
Objective: This study aimed to investigate the protective effect of Silybum marianum (S. marianum) seeds extract its oil fraction against damage effect of γ-radiation in female albino rats.Methods: Ultrasonic-assisted extraction was used for the extraction of S. marianum seeds. Lipid patterns of S. marianum seeds oil were elucidated using gas chromatography-mass spectrometry (GC-MS). S. marianum seeds extract was analyzed using high-performance liquid chromatography (HPLC). Malondialdehyde (MDA), reduced glutathione (GSH) and metallothionein (MT) were estimated in heart and brain tissues of the examined rats. Lactate dehydrogenase (LDH) and creatine kinase-MB (CKMB) were measured in the serum of the examined rats, and the brain biomarkers; dopamine and serotonin were also measured.Results: The oil was found to be rich in linoleic acid (58.20%) and arachidic acid (23.38%). S. marianum seeds extract revealed the presence of taxifolin and six main active constituents of silymarin, including silydianin, silychristin, silybin A, silybin B, isosilybin A and isosilybin B. Treatment of γ-radiation damage effect using S. marianum seeds extract and its oil fraction led to a significant reduction of MDA levels in heart (139.6 and 165.5 nmol/g, respectively) and brain (158.5 and 135.2 nmol/g, respectively) tissues, however, significant increase of GSH levels in heart (316.4 and 293 mg/g, respectively) and brain (210.4 and 227 mg/g, respectively) tissues was observed, also a significant increase of dopamine levels (85.27 and 65.74 ng/g, respectively) and MT levels of heart tissues (108.5 and 70.52 mg/g, respectively) was observed.Conclusion: S. marianum seeds extract and its oil fraction showed a protective effect against γ-radiation-induced damage in heart and brain.Â
Authors and Affiliations
Nohair H. Sherif, Asrar M. Hawas, Walid E. Abdallah, Ibrahim A. Saleh, Khaled A. Shams, Faiza M. Hammouda
Keywords
Related Articles
- EP ID EP575592
- DOI 10.22159/ijpps.2017v9i7.18046
- Views 65
- Downloads 0
WOUND HEALING POTENTIAL OF YOUNG LEAVES OF TRITICUM AESTIVUM ON ALLOXAN INDUCED DIABETIC RATS
Objective: Wheatgrass is known to have several medicinal properties to treat various disorders. Triticum aestivum Linn. is a traditional medicinal plant apparently used for bowel disorders, skin inflammation, infection,...
FORMULATION, OPTIMIZATION, CHARACTERIZATION AND IN VIVO ANTI-ULCER ACTIVITY OF ESOMEPRAZOLE MAGNESIUM TRIHYDRATE GASTRORESISTANT MICROSPHERES
Objective: The objective of the present investigation was to prepare gastro-resistant microspheres of esomeprazole magnesium trihydrate (EMT) to prevent its degradation in the acidic environment of the stomach and enhanc...
AN EXPERIMENTAL STUDY EVALUATING THE INFLUENCE OF BISPHOSPHONATES ON DEPRESSION PATTERNS IN SWISS ALBINO MICE AND WISTER RATS
Objective: Bisphosphonates are used for treating osteoporosis. Few studies have reported their effect on alterations in comorbid behaviour such as depression. Therefore, present study was performed to investigate the eff...
ISOLATION, IDENTIFICATION, AND QUANTIFICATION OF LECTIN PROTEIN CONTENTS IN CHAMERION ANGUSTIFOLIUM L. DRIED RAW MATERIAL AND THE STUDY OF ITS ACTIVITY USING RATUSERYTROAGGLUTINATION
Objective: To isolate, identify and quantify lectin protein contents in Chamerion angustifolium L. dried raw material species namely; leaves in the flowering stage, buds, and flowers and the study of its activity.Methods...
PREPARATION, CHARACTERIZATION AND ANTIOXIDANT ACTIVITY OF XANTHONE-LOADED MAKING (HODGSONIA HETEROCLITA) MICROEMULSIONS
Objective: The aim of this study was to incorporate xanthone into Making (Hodgsonia heteroclita) microemulsions and to evaluate the antioxidant activity of the formulations.Methods: Making oil was obtained from the seed...