Prebiotic effect of Jerusalem artichoke (Helianthus tuberosus) fructans on the growth performance of Bifiobacterium bifium and Escherichia coli
Journal Title: Asian Pacific Journal of Tropical Disease - Year 2016, Vol 6, Issue 5
Abstract
Objective: To investigate and compare in vitro prebiotic effects of Jerusalem artichoke polyfructans on the survivability and activity of Bifiobacterium bifium and Escherichia coli with high performance-inulin (a high molecular weight fraction of chicory-derived inulin). Methods: Extracted polyfructose from Jerusalem artichoke tubers and standard inulin were added to the appropriated culture to achieve final concentrations [0.5%, 1.0%, 2.0% and 3.0% (w/v)] to determine the turbidity and pH variations during 48 h incubation. Results: This study suggested that Jerusalem artichoke tuber fructooligosaccharides had the potential to be used as a prebiotic component. The growth of Bifidobacterium bifidum improved significantly in the presence of Jerusalem artichoke fructans compared to the control. There was no significant differences (P < 0.05) in Bifidobacterium population in different concentrations of Jerusalem artichoke poly-fructans, but the population was significantly higher than the count in the presence of high performance-inulin. The pH decreased in both media during 48 h incubation time. The specific rate of growth and doubling time determined for Escherichia coli demonstrated that the efficacy of various carbon sources in stimulating bacterial growth was influenced by the concentration and degree of polymerization of fructan chains in the media. Conclusions: Jerusalem artichoke fructooligosaccharides can provide the greater stability of probiotics and acid production, so it can be considered as a potential source of high yielding oligosaccharide for commercial prebiotic production to develop food industry and improve host health.
Laboratory rearing of Cimex hemipterus F. (Hemiptera: Cimicidae) feeding on different types of human blood compositions by using modifid artifiial feeding system
Objective: To investigate the effects of three types of human blood compositions: whole blood, red blood cells and red blood cells mixed with plasma, and determine the suitable blood source that can be used to feed the b...
Comparison of plasmid and chromosomal omp1 gene-based PCR and two DNA extraction methods for diagnosing Chlamydia trachomatis in endocervical swab samples
Objective: To evaluate the effectiveness of boiling and proteolytic DNA extraction methods and also to compare the sensitivity of plasmid polymerase chain reaction (PCR) and chromosomal omp1 gene PCR for genital Chlamydi...
In vitro antibacterial potential of Hydrocotyle javanica Thunb.
Objective: To explore the in vitro antimicrobial potential of Hydrocotyle javanica (H. javanica) Thunb. against human pathogenic bacteria and to standardize purification protocol to harvest the active principle in large...
Molecular and cytogenetic evaluation for potential genotoxicity of hydrocortisone
Objective: To assess the risk of hydrocortisone sodium succinate through different end points of genotoxicity. Methods: The study examined the induction of chromosomal aberrations in bone marrow cells, morphological sper...
Assessment of hepatitis B virus and hepatitis C virus infections and associated risk factors in HIV infected patients at Debretabor hospital, South Gondar, Northwest Ethiopia
Objective: To assess hepatitis B and hepatitis C virus infections and associated risk factors among HIV infected patients at Debretabor hospital. Methods: A cross-sectional study was conducted among HIV/AIDS patients att...