Prediction of Antibiotic Influence by Presence of Some β-lactamase Gene in Acinetobacter Infected Patients Hospitalized in Tehran, Iran
Journal Title: Journal of Applied Environmental and Biological Sciences - Year 2012, Vol 2, Issue 8
Abstract
Acinetobacter baumannii which is gram-negative rod and infecting hospitalized patient often develops multiple drug resistance patterns and causes serious problem in treatment. The aim of this study is to determine the relationship between this serious problem in treatment and Presence of some β-lactamase genes in the Acinetobacter baumannii. 100 isolates of Acinetobacter collected from hospitalized patients in Tehran which were identified at the species level by phenotypical test and confirmed by the presence of oxa 51like. First their resistance to different antibiotics were determined using disk agar diffusion test then the MICs of all resistant isolates determined by Etest .In the second stage we screened all resistance isolates for β-lactamase gene existence by double-disk synergy test and finally prevalence of β-lactamase encoding genes such as IMP-1,SIM-1,OXA-23-like,OXA-24-like and OXA-58-like were determined by PCR. According those tests the most efficient treatment was achieved by Colistin, Gentamicin, Imipenem, Amikacin and Ampicillin-sulbactam. Disk synergy test with 0.1M EDTA determined 23 % of isolates as metalo β-lactamase producers. Prevalence of β- lactamase encoding genes was:4%, 0%, 38%, 32%, 1% respectively. According to this study efficient treatment will be influenced by OXA β-lactamase more than the other genes which have been studied .Therefore using of Correlate drugs should be Cautionly. This is the first report of SIM type among MBL-producing A. baumannii in Iranا
Authors and Affiliations
Javad Dadgar Pakdel, Mohammad Niakan, Iraj Rasooli, Davood Yadegari Nia, Firouz Amani, Sohrab Aghabozorgi
Keywords
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- EP ID EP98687
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Prediction of Antibiotic Influence by Presence of Some β-lactamase Gene in Acinetobacter Infected Patients Hospitalized in Tehran, Iran
Acinetobacter baumannii which is gram-negative rod and infecting hospitalized patient often develops multiple drug resistance patterns and causes serious problem in treatment. The aim of this study is to determine the re...