Prediction of lymph node metastasis risk by M2 tumor associated macrophages in colorectal cancer tissue
Journal Title: Chinese Journal of Clinical Research - Year 2024, Vol 37, Issue 9
Abstract
Objective To investigate the risk of lymph node metastasis (LNM) in colorectal cancer (CRC) based on the M2 macrophage activity biomarker soluble CD163 (sCD163). Methods The clinical data and preoperative blood samples of 152 patients with CRC and 58 patients with colorectal adenoma who received primary surgery in Jingmen Central Hospital from January 2018 to May 2019 were collected. Blood samples were collected the day before surgery, and enzyme-linked immunosorbent assay (ELISA) was used to measure serum levels of sCD163, carcinoembryonic antigen (CEA), and carbohydrate antigen 199 (CA199). Postoperative follow-up was conducted with a cutoff date of April 30, 2024, and the overall survival and disease-free survival were recorded. Results The level of sCD163 in patients without LNM was significantly lower than in those with LNM [(2.79±0.76) mg/L vs (4.25±1.50) mg/L, t=7.958, P<0.01]. Multivariate logistic regression analysis indicated that sCD163>3.50 mg/L was an independent risk factor for LNM in CRC patients (HR=13.973, 95%CI: 5.385-36.259, P<0.05). sCD163>3.50 mg/L had the highest ability to predict lymph node metastasis in CRC patients (AUC=0.742), with a sensitivity of 68.8% and a specificity of 79.7%. There were 35 patients (46.1%) died in the group of sCD163>3.50 mg/L, and 12 patients (15.8%) died in the group of sCD163≤3.50 mg/L. There was a significant difference in overall survival between the two groups (log-rank χ2=15.583, P<0.01). There were 42 patients (55.3%) in the group of sCD163>3.50 mg/L relapsed, and 25 patients (32.9%) in the group of sCD163≤3.50 mg/L relapsed. The difference in recurrence-free survival between the two groups was statistically significant (log-rank χ2=8.368, P=0.004). Conclusion Elevated levels of the biomarker sCD163, which reflects M2 macrophage activity in tissues, are significantly associated with LNM and poor prognosis in CRC patients, and sCD163 may be a potential predictive factor for identifying CRC patients at high risk of LNM.
Authors and Affiliations
ZHU Xiaoxuan, LONG Zhou, CAO Shaohua
Application of pancreatic regenerating protein in early warning and diagnosis of diabetes mellitus
Sodium-glucose cotransporter 2 inhibitors (SGLT-2i) are increasingly utilized in the treatment of type 2 diabetes, especially for their efficacy in weight reduction and improvement of cardiovascular and renal outcomes, a...
Efficacy of different approaches of spinal endoscopy in the treatment of L<sub>4-5</sub> intervertebral disc herniation
<b>Objective</b> To compare the clinical efficacy of two approaches under spinal endoscopy in the treatment of L<sub>4-5</sub> intervertebral disc herniation. <b>Methods</b> A retrospective analysis was performed for 1...
Effects of laparoscopic sleeve gastrectomy on sex hormones in male patients with obesity
"<b>Objective</b> To evaluate the changes in sex hormone levels in male patients with obesity after receiving laparoscopic sleeve gastrectomy (LSG). <b>Methods</b> Forty-three male obese patients who received LSG at th...
Predictive indicators of acute kidney injury caused by exertional rhabdomyolysis
Objective To investigate the characteristics of exertional rhabdomyolysis (ERB) and the predictive value of creatine kinase (CK), myoglobin/creatine kinase (Mb/CK) ratio and other indicators for ERB patients with acute r...
Research progress of programmed cell death in colorectal cancer
Programmed cell death is an important biological process, including apoptosis, autophagy and other modalities, which is essential for maintaining homeostasis of tissue and organ. In recent years, several studies have sho...