PREDICTION OF PROMISCUOUS EPITOPE STUDIES OF SPA ANTIGEN IN STAPHYLOCOCCUS AUREUS: AN INSIGHT ON PEPTIDE-BASED VACCINE
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 7
Abstract
Objective: Owing to the difficulty in providing drug therapies against Methicillin-resistant Staphylococcus aureus (MRSA), the development of an effective and promising vaccine is an immense challenge in combatingMRSA infections. The present work focuses on the development of a peptide-based vaccine, by identifying the epitopes from SPA antigen.Methods: The epitopes were identified based on different properties, such that they are capable of eliciting broadly neutralizing immune responses. The identified epitopes were subjected for peptide docking using Glide and antibody-antigen docking using ClusPro.Results: By in silico approach two epitopes “NLNEEQRNG†and “LKDDPSQSAN†were identified for SPA protein with sequence lengths of nine and ten respectively. The least energy for the peptide docking was observed for NLNEEQRNG sequence and the amino acid residues of this peptide share similar interaction with antibody-antigen docking.Conclusion: Based on the properties and docking studies the best-ranked epitope sequence is ‘NLNEEQRNG’. Further studies on this peptide sequence might be helpful for alternative therapy of MRSA infections.Keywords: Epitope, SPA antigen, IgG antibody, Docking studies
Authors and Affiliations
Kunal Zaveri, Kiranmayi Patnala
Keywords
Related Articles
- EP ID EP576276
- DOI -
- Views 63
- Downloads 0
VALIDATED SPECTROPHOTOMETRIC METHOD FOR THE ESTIMATION OF VINCRISTINE AND VINBLASTINE
Objective: A simple, sensitive, precise, reproducible and validated UV spectrophotometric methods have been developed for the determination of vincristine (VCR) and vinblastine (VLB) in the pure and dosage forms.Methods:...
AN OVERVIEW OF RECOMBINANT VACCINE TECHNOLOGY, ADJUVANTS AND VACCINE DELIVERY METHODS
Development of an effective vaccine is of paramount important in disease prevention and control. As such, recombinant technology can serve as a gateway for the development of safe and effective vaccines that can be deliv...
ESTIMATION OF ETONOGESTREL IN HUMAN PLASMA BY USING LC–ESI–MS/MS METHOD
Objective: The aspiration of the present study was to develop simple, robust and reliable liquid chromatography/electro spray ionization tandem mass spectrometry (LC-MS/MS) (Agilent Technologies) assay method for the qua...
ASSESSMENT OF FREE RADICAL SCAVENGING ACTIVITY OF TEN MADHURASKANDHA DRUGS THROUGH UV SPECTROSCOPIC AND CHROMATOGRAPHIC TECHNIQUE
Objective: Acharya Charaka had classified Asthapana bastidravya (corrective enema) based on Rasa (Taste), called as Rasaskandha (a group of drugs having similar taste). He ascertained some criteria to include drugs in th...
ANTIDIABETIC STUDY OF COMBINATION OF ANDROGRAPHIS PANICULATA (BURM. F.) WALLICH. EX NEES. HERBS EXTRACT AND GUAZUMA ULMIFOLIA LAMK. LEAVES EXTRACT IN OBESE DIABETIC MICE MODEL
Objective: This research was to investigate the activity of Andrographis paniculata extract (APE) and Guazuma ulmifolia extract (GUE) in treating obesity integrated diabetic conditions.Methods: There were two stages of i...