Preliminary identification of CD4 T cell epitopes in genotype 1 and 6 of hepatitis C virus
Journal Title: Chinese Journal of Blood Transfusion - Year 2022, Vol 35, Issue 7
Abstract
Objective To study the CD4 T cell epitopes in Core and NS3 protein of genotype 1(GT1) and 6(GT6) of hepatitis C virus(HCV). Methods A total of 298 overlapping peptides(16-mer) spanning Core and NS3 protein of GT1 and GT6 HCV were synthesized. Peripheral blood mononuclear cells(PBMCs) from 17 HCV+ and 7 healthy blood donors were stimulated by peptide pools, followed by evaluating T cell response by IFN-γ ELISPOT, by which 21 peptides with positive results were found. These peptides were further applied to individually stimulate 20 HCV+ and 18 healthy PBMCs. The differences of responsive frequencies to the 21 positive peptides between the two study groups were compared. Results Pooled and individual peptide stimulation tests showed that HCV+ PBMCs were responsive to the stimulation of 5 peptides(GT1 NS31273-1288 and NS31315-1330; GT6 NS31033-1048, NS31087-1102 and NS31351-1366), with a responsive frequency ranging 18.9%-27.0%. In contrast, healthy PBMCs were not or low responsive(0%-4.0%) to these five peptides. The responsive frequencies were statistically different between the two groups(P<0.05). No reported epitopes in IEDB were found identical with these 5 peptides via sequence alignment. Conclusion Our study identified novel CD4 T cell epitopes in NS3 protein of GT1 and GT6 HCV, which has potential application value for the research and development of HCV vaccine.
Authors and Affiliations
Jieting HUANG, Qingzhu YOU, Fengpei LI, Ru XU, Min WANG, Qiao LIAO, Zhengang SHAN, Xia RONG, Yongshui FU
Keywords
Related Articles
- EP ID EP738283
- DOI 10.13303/j.cjbt.issn.1004-549x.2022.07.003
- Views 27
- Downloads 0
The challenge of finding Chinese blood groups
Up to now, hundreds of human blood groups have been detected globally, but none have been found in the Chinese population. China is a multi-ethnic country with rich genetic polymorphism and variation. The Chinese pangeno...
Inhibitory effect of Salidroside on the interaction between human platelets and breast cancer MDA-MB-468 cells
Objective To study the effect of Salidroside(Sal) on platelet activation and aggregation and the interaction between human platelets and MDA-MB-468 cells of breast cancer. Methods Human platelets were collected, platelet...
The influence of scene dialogue on the experience of blood donors during blood donation
Objective To explore the effect of repeated use of situational dialogues in the process of blood donation of voluntary blood donors. Methods From September to November 2019, blood donors in the center were investigated i...
Donor plasma reinfusion flushing of the LRS chamber to prevent CD4+ and CD8+ T lymphopenia
Objective To explore the effectiveness of using donor plasma reinfusion to flush the leukoreduction system (LRS) chamber during the final reinfusion phase with the Trima Accel automated blood collection system in prevent...
Effect of MAP on preservation quality of deglycerolized red blood cells
Objective To observe the effect of deglycerolized red blood cells suspended in MAP on preservation and explore the most effective preservation method. Methods Concentrated red blood cells were prepared by centrifuging 40...