Preliminary studies on the usefulness of DNA mini-barcodes for determining phylogenetic relationships within shieldbugs (Hemiptera: Heteroptera: Pentatomidae)
Journal Title: Heteroptera Poloniae - Acta Faunistica - Year 2012, Vol 4, Issue
Abstract
The paper presents results of preliminary studies on the usefulness of DNA mini-barcodes for the phylogenetic inference within Pentatomidae. Mini-barcodes for six Polish shieldbug species representing three pentatomid subfamilies (Asopinae, Pentatominae, Podopinae) have been obtained from the museum specimens (collected between 1994-2004). These mini-barcodes were then analysed for generating phylogenetic trees, using the neighbor joining (NJ), minimum evolution (ME), maximum parsimony MP), and UPGMS methods, as well as the Bayesian inference (BI). A squashbug, Coreus marginatus (Coreidae) was treated as an outgroup species in all analyses. Our findings suggest that DNA mini-barcodes can be employed not only for species identification, but may also be used for the phylogenetic inferences.
Authors and Affiliations
Jerzy A. Lis, Joanna Olchowik, Magdalena Bulińska-Balas
Keywords
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