Preparation and evaluation of alginate nanoparticles containing pertussis toxin as a particulate delivery system

Journal Title: International Journal of Advanced Biotechnology and Research. - Year 2016, Vol 7, Issue 2

Abstract

ABSTRACT During the last decades, research on different kinds of nanoparticles (NPs) has been increased to prepare various medical applications, for instance in vaccine and gene delivery and as new generation of adjuvant candidates. The aim of the present study was to prepare sodium alginate nanoparticles (Alg-NPs) containing pertussis toxin (PTX) as an candidate acellular vaccine. Formulation of antigen loaded Alg-NPs were assessed for immunological activities and their role as potential immunological adjuvant. Alg-Nps were prepared using mild ionic gelation method. Optimal formulation was obtained by concentration of 0.2%w/v sodium alginate, 0.1%w/v CaCl2 solution and magnetically homogenization condition of 45 min and rate of 2000 rpm. Obtained Alg-NPs showed average size of 88 nm and zeta potential of -32mV in blank and 72 nm and -29 mV in PTX entrapped nanoparticles, respectively. A Loading efficiency of more than 90% was determined for PTX. The antigen loaded nanoparticles showed 75.3% of release within 144 h in in vitro release studies. The immunological evaluation in female Balb/c mice groups revealed that the Alg-NPs formulation induced significantly higher serum antibody titers (p < 0.01) as compared with commercial acellular pertussis vaccine and conventional alum-adjuvanted antigen administered by subcutaneous route. The results showed the potential of Alg-NPS to be a simplex and efficient delivery system. This study also indicated the potential of Alg-NPs as new generation of immunostimulant adjuvant to boost the antigenecity of the antigens in a cellular pertussis vaccines.

Authors and Affiliations

Hamid Reza Goudarzi1, A. R. Mokarram1*, M. Noofeli1, A. N. Shirvan1 and M. Saadati2

Keywords

Alginate Nanoparticles; Pertussis toxin; Vaccine delivery; Adjuvants

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