Preparation of Fowl Typhoid Vaccine from Field Isolates and Determination of Efficacy
Journal Title: Microbiology Research Journal International - Year 2016, Vol 14, Issue 1
Abstract
The experiment was conducted to isolate and identify Salmonella gallinarum from field cases to prepare formalin killed vaccine and to determine the efficacy of experimentally prepared fowl typhoid vaccine. A total of 48 chickens were divided into six groups (group A, B, C, D, E and group of unvaccinated control chickens F) including 8 layer chickens of Sonali breed in each group. Chickens in group A, B, C, D and E were vaccinated primarily with experimentally prepared fowl typhoid vaccine with a dose 0.5 ml (4.7 × 107 CFU/ml) through subcutaneous route at the age of 9 weeks and booster dose at 14, 21, 28, 35, 42 days after primary vaccination with the same dose and route respectively. Blood samples were collected to obtain sera from each chicken after 15 days boostering for determination of antibody titre following using passive haemagglutination test. Highest mean antibody titres obtained from Group A, B, C, D and E was 96±12.04. Among the five groups the highest mean antibody titre of 96±12.04 was obtained when vaccine was given at 14, 21, 28 days after primary vaccination. The result of Challenge infection revealed that among the 8 birds of A, B, C, D and E all were protected from virulent challenge and all chickens were died from the group F. These results revealed that experimentally prepared Fowl typhoid vaccine provided 100% protection.
Authors and Affiliations
Jannatun Nime, Mehedi Hasan, Md. Abdus Sattar, Mst. Dilara Nuri, Md. Bahanur Rahman, Afia Khatun
Keywords
Related Articles
- EP ID EP350834
- DOI 10.9734/BMRJ/2016/24994
- Views 64
- Downloads 0
Aeromycoflora of Animal Rearing Houses of Bangalore, India
The present study was carried out to investigate the indoor and outdoor airborne fungi in the animal rearing houses viz., rabbit house, cow shed, poultry farm and swine house in Hessaraghatta village, Bangalore city was...
Impact of Endomycorrhizae and Acidithiobacillus ferrooxidans with Sulfur and Phosphorus Nutrition on Onion (Allium cepa L.) and Maize (Zea mays L.) Plants under Field Conditions
Aim: To evaluate the effects of endomycorrhizal fungi (AMF) and sulfur oxidizing bacteria as biofertilizers in presence of phosphors and sulfur as mineral nutrients on onion (Allium cepa L.) and maize (Zea mays L.) plant...
Continued Circulation of DENV-2 (Genotype IV) in Delhi, India
Background: Dengue is one of the rapidly emerging arboviral infection in many parts of the world including India. The metropolitan city, Delhi is one of the worst affected areas by dengue. In the last two decades, it has...
Potential Infections Linked to the Microbiological Quality of Swimming Pools _Kumasi, Ghana, West Africa
The risk of infections associated with microbiological quality of swimming pools in Kumasi was investigated. A variety of microorganisms can be found in swimming pools and similar recreational water environments which ma...
Assessment of Bacteria and Heavy Metals Contamination in Lettuce at Farm Gate and Market in the Accra Metropolis
The bacteria and heavy metals contamination of lettuce from two vegetable growing farms irrigated with either stored municipal, stream or polluted drain water were assessed on farm and in the market. A total of 120 irrig...