Preparation of psyllium husk powder based microporous composite scaffolds for tissue engineering
Journal Title: Engineering of Biomaterials / Inżynieria Biomateriałów - Year 2018, Vol 21, Issue 147
Abstract
This study demonstrates the comparison in the method of fabrication and thus evaluates the potential of psyllium husk powder and gelatin-based composite microporous scaffolds for tissue engineering applications. The scaffold is being prepared in three different ratios of 50:50, 75:25 and 100 (w/w of psyllium husk powder and gelatin, respectively) by employing a suitable cross-linking agent, EDC-NHS, followed drying. We have demonstrated the use and outcomes of two different methods of scaffold drying, i.e., vacuum desiccation along with liquid nitrogen dip and lyophilization. It was concluded from the SEM micrographs that the scaffolds dried under vacuum accompanied with liquid nitrogen exposure exhibited less porous architecture when compared to those prepared using a lyophilizer, that resulted in pores in the range of 60-110 μm. Scaffolds fabricated using the former technique lost porosity and sponge-like characteristics of a scaffold. In spite of the above fact, water retaining capacity and stability in the cell culture of such scaffolds is significant, nearly 40-50% of its initial dry weight. Cell culture experiments support the potential of the scaffolds prepared from different methods of fabrication for its cytocompatibility and suitability for cell growth and proliferation for a substantial duration. Erosion in the porous design of the scaffolds was observed after 14 days via SEM micrographs. It was inferred that freeze-drying is a better technique than vacuum desiccation for scaffold preparation. The present investigation has been conducted keeping in mind the importance of drying a scaffold. Scaffold drying is a necessary step to increase its shelf-life, makes it easy to transport and much importantly, controlling the pore size of the scaffold.
Authors and Affiliations
S. Poddar, P. S. Agarwal, S. K. Mahto
Keywords
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