Prevalence and Evaluation of Antimicrobial Activity of Dodonaea viscosa Extract and Antibacterial Agents against salmonella Spp. Isolated from Poultry
Journal Title: Scholars Academic Journal of Biosciences - Year 2014, Vol 2, Issue 12
Abstract
A total of 200 samples (180 fecal materials and 20 organ samples) were collected from (5 different poultry farms, 10 local poultry shops, 5 houses poultry, 5 Eggs stores shops and 5hand slaughters centers) in Ibb city, Yemen, 2014. According to morphological, cultural, as well as biochemical characterization and serological tests, 59(29.5%) isolates were identified as Salmonella spp. and all Salmonella isolates were categorized by serotype, which comprised of, 37(62.71%) Salmonella Typhimurium serovar, 21(35.59%). Salmonella Enteritidis serovar and 1(1.69%) Salmonella Heidlberg serovar. Antibiotic sensitivity test was done for bacterial isolates and the results showed there were clear differences in antibiotic resistant. Antimicrobial susceptibility of the isolates varies as follows: Ofloxacin (79.66%), Ciprofloxacin (67.80%), Colistin (59.32%) and Gentamycin (52.54%). All of isolates were resistant to Erythromycin, Penicillin and Lincomycin. Antibacterial activity was done for both aqueous and ethanol extracts of Dodonaea viscosa plant by using well and disc diffusion assay. The results indicated that well diffusion assay had best results than disc diffusion assay, the highest inhibition zone was (22)mm for well diffusion and (15)mm for disc diffusion assay, the results observed that ethanol extract had best antibacterial effect than aqueous extract which the percentage of bacterial isolates affected with ethanol extract was(71.19%) comparing with aqueous extract (28.81%)by using disc diffusion assay, while the percentage of bacterial isolates affected with ethanol extract was(88.13%) comparing with aqueous extract (52.54%)by using will diffusion assay.
Authors and Affiliations
Shayma Munqith Al-baker, Fadhl Ahmed Saeed Al-gasha’a, Samira Hamid Hanash, Ahmed Ali Al-Hazmi
Bioactive Exopolysaccharides (EPS) Synthesized From Exiguobacteriumaurantiacum and Brevundimonasdiminuta with Myeloid Cancer Cells Inhibiting and Flocculating Activity
Exopolysaccharides (EPS) are polysaccharides secreted by certain bacteria. Exiguobacteriumaurantiacum and Brevundimonasdiminuta, isolated from Nador’sMarchica lagoon from soil and water samples were found to be capable...
Biosynthesis and Characterization of Different Metal Nanoparticles by Using Fungi
The biosynthesis of metal nanoparticles has become a matter of great interest in recent times due to their various advantageous properties and applications in a variety of fields. The exploitation of different fungi for...
Saliva – A Diagnostic Tool in Assessment of Lipid Profile
Cholesterol, a crucial substance that performs innumerable vital functions in the body, has also been considered as a risk factor for various pathological states including atherosclerosis. Saliva cholesterol concentratio...
Physiological Changes in the Venous System of Lower Extremities in a Case of Varicose Vein
Varicose vein is a common clinical entity, with their symptoms and the most common complication like chronic peripheral vascular disorders. In this study, a 45 yrs old male, presented with a complaints of ulcer in the la...
Pathogenicity and Virulence Factors of Phytobacteria
Plant pathogenic bacteria have evolved specialized strategies to exploit their respective hosts. Most of them are Gram-negative, of which biotropic pathogenic bacteria fundamentally possess a type III secretion system en...