Prevalence and Molecular Characterization of Shiga-toxigenic Escherichia coli in Piglets of North East Region of India
Journal Title: International Journal of TROPICAL DISEASE & Health - Year 2017, Vol 27, Issue 2
Abstract
Background: Shiga-toxigenic E. coli also known as verotoxin–producing E. coli is one of the diarrhoeagenic E. coli strains which also include its well-known subgroup enterohaemorrhagic E. coli. It is increasingly recognized as the cause of severe gastrointestinal and systemic diseases, such as haemorrhagic colitis and haemolytic uraemic syndrome. Materials and Methods: The present research study was conducted during 2013-15 to investigate the prevalence and molecular characterization of Shiga-toxigenic Escherichia coli associated with gastroenteritis of piglets in organized and unorganized farms of North East Region of India. A total of 457 faecal samples were collected from unweaned piglets in organized and unorganized farms of North East States of India. All the isolates were screened by multiplex PCR assay for presence of putative virulence genes (stx1, stx2, and hlyA), serotyped and further characterized for resistance against 15 selected antimicrobial drugs. Results: Of the 1286 E. coli isolates screened by multiplex PCR, a total of 30 isolates (2.33%) were recorded as STEC and were isolated from diarrhoeic piglets only. Of the 30 STEC, 28 isolates were positive for stx2 gene and 2 isolates possessed hlyA gene. Nineteen STEC (2.9%) were recovered from organized farms and 11 (1.74%) from unorganized farms, further, 5 STEC isolates (1.51%) were isolated from local breed and 25 (2.61%) from cross breed piglets. The 30 STEC isolates belonged to 9 different serogroups, 4 isolates (13.33%) were untypable and 5 isolates (16.66%) were rough strains. Serogroups, O1, O8, O156 were found to be the most prevalent serotype (10.0%). All the isolates showed resistance to at least three antimicrobial agents. None of the isolates were found resistance against imipenem, whereas the highest resistance was observed against cefalexin (80.0%) and amoxycillin (76.66%).
Authors and Affiliations
Hosterson Kylla, Tapan Kumar Dutta, Lallianpuii Kawlni
Keywords
Related Articles
- EP ID EP312793
- DOI 10.9734/IJTDH/2017/37109
- Views 62
- Downloads 0
A Five Year Retrospective Study of Vascular Tumours at Federal Medical Center Makurdi and Benue State University Teaching Hospital
Aim: To determine the prevalence of vascular tumours, and their morphology at Federal Medical Centre and Benue State University Teaching Hospital Makurdi. Study Design: Retrospective study of vascular tumours at the two...
Prevalence and Factors Associated with Opportunistic Infections in HIV Positive Patients on Antiretroviral Therapy in Uganda
Introduction: Opportunistic infections (OIs) remain the single main cause of ill-health and death among HIV/AIDS patients in resource poor countries. We assessed the prevalence of 17 OIs and associated factors among HIV...
Implementing Surveillance and Outbreak Response Management and Analysis System (SORMAS) for Public Health in West Africa- Lessons Learnt and Future Direction
Background: Mobile devices have the potential to strengthen surveillance in sub-Saharan Africa. Electronic surveillance (eSurveillance) is the use of electronic systems to facilitate public health surveillance. Mobile he...
Comparison of School Health Services among Private and Government Owned Schools of Belgaum
Introduction: Children are the creators and the shapers of tomorrow and school marks an important early milestone in the child’s life long journey of intellectual and psychological development. Objective: To assess the...
Nocardiosis –A Series of Four Case Reports
Aim: Nocardia spp are ubiquitous soil organisms that often infect patients with underlying compromised immunity, pulmonary disease or history of surgery or trauma. The diagnosis of nocardiosis can be missed because there...