Prevalence of Biofilm Formation and Wide Distribution of Virulence Associated Genes among Vibrio spp. Strains Isolated from the Monastir Lagoon, Tunisia
Journal Title: Polish Journal of Microbiology - Year 2016, Vol 65, Issue 3
Abstract
In the current study, 65 Vibrio spp. were isolated from the Monastir lagoon water, were characterized phenotypically and genotypically. In addition, we looked for the presence of three Vibrio parahaemolyticus virulence genes (tlh, trh and tdh) and ten Vibrio cholerae virulence genes (ctxA, vpi, zot, ace, toxR, toxT, tosS, toxRS, tcpA and cpP). We also investigated the antibiotic susceptibilities and the adherence ability of the identified strains to abiotic material and to biotic surfaces. The cytotoxicity activity against HeLa and Vero cell lines were also carried out for all tested strains. All Vibrio isolates were identified to the species level and produced several hydrolytic exoenzymes. The results also revealed that all strains were expressing high rates of resistance to tested antibiotics. The minimum inhibitory concentration (MIC) values showed that tetracycline and chloramphenicol were the most effective antibiotics against the tested bacteria. Vibrio alginolyticus and V. cholerae species were the most adhesive strains to both biotic and abiotic surfaces. Besides, V. alginolyticus isolates has the high levels of recombination of genes encoding V. cholerae and V. parahaemolyticus virulence factors. In vitro cytotoxic activities of several Vibrio extracellular product were also observed among HeLa and Vero cells.
Authors and Affiliations
Badreddine Mechri, Amel Medhioub, Mohamed N. Medhioub, Mahjoub Aouni
Keywords
Related Articles
- EP ID EP81126
- DOI 10.5604/17331331.1215610
- Views 76
- Downloads 0
The probable interspecies transfer of the bla<sub>VIM-4</sub> gene between Enterobacter cloacae and Klebsiella pneumoniae in a single infant patient
We report interspecies transfer of the [i]bla[/i][sub]VIM-4[/sub] gene in MBL-producing [i]Enterobacter cloacae[/i] and Klebsiella pneumoniae isolates from a newborn patient who had received meropenem therapy. We sh...
Molecular Characterization of Shiga Toxin-Producing Escherichia coli Strains Isolated in Poland
Shiga toxin-producing Escherichia coli (STEC) strains also called verotoxin-producing E. coli (VTEC) represent one of the most important groups of food-borne pathogens that can cause several human diseases such as hemorr...
Identification and Localization of β-D-Glucosidase from Two Typical Oenococcus oeni Strains
β-D-glucosidase (βG) gene from [i]Oenococcus oeni [/i]SD-2a and 31MBR was cloned, sequenced and analyzed, also intracellular βG of the two strains was further localized. Results showed that βG gene of the two strains was...
Expression of the Fluoroquinolones Efflux Pump Genes acrA and mdfA in Urinary Escherichia coli Isolates
Escherichia coli is one of the most frequent causes of urinary tract infections. Efflux system overexpression is reported to contribute to E. coli resistance to several antibiotics. Our aim in this study was to investiga...
The Prevalence of Campylobacter spp. in Polish Poultry Meat
The prevalence, count and molecular identification of Campylobacter spp. in Polish poultry meat were analysed. 181 samples of meat from chicken (70), turkey (47), duck (54) and goose (10) were studied. Campylobacter spp....