Prevalence of Candida Species Isolated from Vaginal Discharge of Women Undergoing in vitro FertilizationEmbryo Transfer in Vietnam
Journal Title: Biomedical Journal of Scientific & Technical Research (BJSTR) - Year 2019, Vol 14, Issue 5
Abstract
Purpose: The aim of this study was to find the prevalence of Candida species isolated from vaginal discharge of women undergoing IVF-ET in Vietnam. Materials and Methods: 83 strains isolated from women undergoing IVF-ET in Military Institute of Clinical Embryology and Histology were collected between November 2017 and December 2018. 83/83 strains were identified as Candida species by several phenotypic methods (i.e., germ tube test, culture on Brilliance™ Candida Agar) and molecular (PCR-RFLP and sequencing). Results: The results showed that, C. albicans (n = 33, 39.76%) was the most common species, followed by C. glabrata (n = 32, 38.56%), C. tropicalis (n = 9, 10.84%), C. krusei (n = 7, 8.43%), C. parapsilosis (n = 2, 2.41%), respectively. Conclusion: In the present study, the most common species in women undergoing IVF-ET were C. albicans, C. glabrata and C. tropicalis, respectively. The frequency of the non-albicans Candida species were increasing, especially C. glabrata. Vulvovaginal candidiasis is estimated to be the second most common cause of vaginitis after bacterial vaginosis [1]. Many studies have shown that approximately 75% of healthy female population at least one symptomatic episode of vulvovaginal candidiasis (VVC) and 40-50% will have recurrent episodes during their lifetime [2,3]. Candida albicans was accounted as the first vulvovaginal candidiasis causative agent [1], followed by non-albicans Candida (NAC) species including Candida glabrata, Candida parapsilosis, Candida tropicalis and Candida krusei [4]. The species distribution of Candida isolates varies between countries, regions, and institutions [5]. NAC species have increasingly been identified as the cause of vulvovaginitis [6]. The data of some reports indicated that NAC species were responsible for 10% to 30% of episodes in certain geographic regions [6]. In clinical practice, the yeast identification is based on morphological and biochemical markers, including the automated methods [7]. However, not all the species are precisely identified by such procedures. Therefore, molecular markers were utilized in the present study to enable identification and detection of various strains [8]. According to the literature, identification of the Candida isolates is important for treatment, prediction of the prognosis, performing infection control, finding epidemiological data and avoid antifungal failure [9,10]. In addition, the species identification of the yeast should be performed with a high accuracy [11]. Previously, very limited data is available on species composition of Candida in Vietnam, especially the women undergoing IVF-ET. This study has been performed to find the prevalence of Candida species isolated from vaginal discharge of women undergoing IVF-ET in Vietnam.
Authors and Affiliations
Do Ngoc Anh, Nguyen Duy Bac, Le Tran Anh, Le Bach Quang, Nguyen Khac Luc, Trinh The Son, Hoang Anh Tuan, Do Quyet
Keywords
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- EP ID EP594777
- DOI 10.26717/BJSTR.2019.14.002611
- Views 138
- Downloads 0
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