Prevalence of Onychomycosis in Hail Region, Saudi Arabia
Journal Title: National Journal of Laboratory Medicine - Year 2018, Vol 7, Issue 2
Abstract
Introduction: Dermatophytes, yeast and non-dermatophyte molds causes onychomycosis, which is the fungal infection of the nail bed and skin beneath, have four clinical types: Distal subungual, proximal subungual, superficial and total dystrophic. Aim: Isolation and identification of causative agents by conventional and molecular methods in order to examine the relative prevalence of dermatophytes, yeast and non dermatophyte molds among patients and correlate the clinical pattern to the agents. Materials and Methods: From dermatology private clinics, beauty salons and female students of Hail University, adults(15- 70 years old) crusts and nail clipping particles of 450 nail samples were collected from toe nails and finger nails in sterile petri dishes. Direct microscopic examination was applied with KOH 20% and gentle heating. Culture, API 32 C® and PCR-ITS, PCR-RFLP were carried out. Results: Positive cases for fungal elements were 296 (65.8%), DLSO were the major type (50.6%), TDO (31.5%), PSO (10.5%) and SWO (1.7%). As causative agents Candida albican was 33.1%, Trichophyton rubrum 18.2%, Trichophyton mentagrophytes complex 16.9%, mixed infection 14.5%, Epidermophyton floccosum 9.5% and Aspergillus niger was 7.8%. Conclusion: Dermatophyteic onychomycosis were mostly found followed by yeast and non dermatophyte molds. Accurate identification of the causative agents help managing onychomycosis by giving the correct antifungal treatment, patients awareness prevent relapse and malformation of nails.
Authors and Affiliations
Soha Abdallah Moursi, Razan Ahmed Al Baqawi, Manal Naif Alshammari, Najah Shulaywih Al Enzi
Keywords
Related Articles
- EP ID EP528922
- DOI 10.7860/NJLM/2018/35007:2283
- Views 130
- Downloads 0
Time and Motion Study of Blood Delivery System in a Regional Blood Transfusion Centre in West Bengal: A Pilot Study
Introduction: Delay in turnaround time (TAT) of blood delivery for transfusion is an important administrative problem in hospitals. Time and motion study for a definite root cause analysis (RCA) is valuable to identify t...
Breast Hamartoma: A Rare Case Report
ABSTRACT Hamartomas of the breast are rare benign lesion which are often seen in females more than 35 years of age. Here, we present a case report of a 32-year-old female with progressively enlarging left sided breast lu...
Skin Appendageal Tumours: An Institutional Experience from Northern India
Introduction: Skin Appendageal Tumours (SAT) are a heterogenous group of rare tumours having differentiation towards the skin appendageal structures and are rarely considered clinically as a differential diagnosis. A cou...
Evaluation of Diagnostic Efficacy of Two Different Microscopic Techniques and Fungal Culture in Onychomycosis
Introduction: Onychomycosis is a term used to describe any fungal infection of nail apparatus and is caused mainly by dermatophytes, non-dermatophytic moulds and yeast. Clinical examination based on physical findings alo...
Comparison of Amsel’s Criteria, Nugent Score and Culture for the Diagnosis of Bacterial Vaginosis
Introduction: Bacterial vaginosis is defined as an alteration in the vaginal microbiota that presents as foul smelling vaginal discharge. This syndrome not only leads to various adverse outcomes of pregnancy, but also in...