Prevalence of Staphylococci in Commercially Processed Food Products in Karachi-Pakistan
Journal Title: Journal of Microbiology and Infectious Diseases - Year 2017, Vol 7, Issue 2
Abstract
Objectives: This study was designed to determine the prevalence of Staphylococci, Staphylococcus aureus (S. aureus), and Methicillin Resistant S. aureus (MRSA) in commercially prepared food items that involve human handling, in Karachi-Pakistan. Methods: In a cross-sectional survey approach, in total 1012 food samples were analyzed. These samples were provided by different food processing industries during 2013 to 2016. Barid-Parker agar plates with egg yolk tellurite (Oxoid) along with Mannitol Salt Agar (BioM), Staph-chromo agar (Merck), Staphylococcus 110 Agar (BioM), and Blood Agar (Oxoid) were used for isolation and identification of Staphylococci. Polymerase chain reaction (PCR) was used for amplification of mecA gene and specie identification via 16s RNA. Results: Total 723 samples (71.4%) showed the presence of Staphylococci. Out of 723 staphylococcal isolates, 367 (36.2%) were S. aureus and 85 (8.3%) isolates were confirmed as MRSA. Molecular studies for MRSA typing showed that most of the isolates (74.1%) belong to SCCmecA IV and 20% MRSA isolates were SCCmecA type II and 5.8% isolates carried SCCmecA type III. The MRSA isolates of SCCmecA type II belong to agr type I, while majority (67%) of the isolates carry agr type II. Conclusion: This study suggested that majority of MRSA isolates recovered from commercial food items belongs to SCCmecA IV and human handling is a major factor for introduction of staphylococci in processed food items. J Microbiol Infect Dis 2017; 7(2): 83-87
Authors and Affiliations
Manzoor Ali Mirani, Afsheen Aqeel, Shagufta Naz, Anila Siddiqi, Mohammad Naseem Khan, Seema Ismat Khan
Keywords
Related Articles
- EP ID EP413370
- DOI 10.5799/ jmid.328789
- Views 118
- Downloads 0
A Cervical Mass in an HIV-Positive Patient
We describe an HIV-infected man with a left side cervical swelling which finally diagnosed with tuberculosis.In contrast to HIV-negative persons, nearly all tuberculous lymphadenitis (scrofula), the most kind of extrapul...
Occupational exposures among healthcare workers: A teaching hospital sample
Objective: Healthcare workers (HCWs) are at risk for occupational injury associated with contaminated blood and body fluids. This study aims to examine the frequency and type of occupational injuries and to determine bes...
Utilization of Alpha-1 Anti-trypsin (A1AT) in Infectious Disease Monitoring and Treatment
Alpha one anti-trypsin (A1AT) is a major serine protease inhibitor found circulating in human blood. A1AT related studies mainly focus on A1AT potential biomarker as well as therapeutic target in non-infectious diseases....
Molecular detection of aac(6’)-Ib-cr among clinical Enterobacterial isolates conferring quinolone resistance:- A study from North east India
Objective: Fluoroquinolones are the commonly prescribed antimicrobial for Gram-negative and Gram-positive mi-croorganisms. The resistance against these antibiotics is of importance in therapy. The current study aimed at...
Miliyer tüberküloz menenjit için ipucu olabilir mi? 25 erişkin miliyer tüberkülozlu menenjit olgusunun değerlendirilmesi
Amaç: Bu çalışmada, miliyer akciğer tüberkülozu (TB) olan ve olmayan tüberküloz menenjitli (TBM) olguların özelliklerinin karşılaştırılması amaçlanmıştır. Yöntemler: TBM’li 160 olgudan miliyer akciğer TB’si olan 25 olgu...