PREVALENCE OF SUB - CLINICAL MASTITIS IN AREAS AROUND LAKHIMPUR TOWN OF ASSAM
Journal Title: International Journal of Agricultural Science and Research (IJASR) - Year 2017, Vol 7, Issue 1
Abstract
Mastitis poses a serious threat to the economics of the dairy industry since it affects both the quality and the quantity of milk produced. The subclinical form can be more challenging since it remains largely undiagnosed, persists for longer duration and is more prevalent than the clinical form. The present study, therefore, was undertaken to assess the prevalence of subclinical mastitis in local and cross bred cows of unorganized farms located in and around North Lakhimpur town of the state of Assam. Milk samples from apparently mastitis free 368 quarters of 92 cows belonging to different herds were collected for this study following standard protocol. The samples were subjected to California Mastitis Test (CMT), Modified Whiteside Test (MWT) and pH test. The pH meter readings of the milk samples could not yield any conclusive difference between the samples. The samples exhibiting gel formation in CMT and MWT were considered as positive for subclinical mastitis. A total number of 18 (19.56 %) cows were found to be CMT positive with all the four quarters showing positive reaction. On quarter basis, 72 (19.56 %) quarters were found to be affected. Similar results were obtained using both the tests. Primary isolation from the positive samples were done on Blood agar followed by subculture on Mac Conkey agar, Eosin Methylene Blue Agar and Baird Parker Agar plates, supporting the growth of various types of bacteria for their study and isolation. Bacterial identification was based on their morphology and motility, growth characteristics on the culture medium and biochemical characteristics. Out of the 18 positive samples Klebsiella pneumoniae could be isolated from 11 samples (61.11 %), followed by Escherichia coli from 7 samples (38.88%), coagulase negative Staphylococcus spp. from 5 samples (27.77%) and Proteus spp. from 3 (16.66%) .
Authors and Affiliations
SOPHIA M. GOGOI, UMA RAM TAMULY, LAISHRAM SANATHOI KHUMAN, GIRINDRA KUMAR SAIKIA, GAUTAM BORDOLOI
Keywords
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