Prevention of γ-Radiation-Induced DNA Damage in Human Lymphocytes Using a Serine-Magnesium Sulfate Mixture
Journal Title: Sultan Qaboos University Medical Journal - Year 2017, Vol 17, Issue 2
Abstract
Objectives: Ionising radiation has deleterious effects on human cells. N-acetylcysteine (NAC) and cysteine, the active metabolite of NAC, are well-known radioprotective agents. Recently, a serine-magnesium sulfate combination was proposed as an antidote for organophosphate toxicity. Tis study aimed to investigate the use of a serine-magnesium sulfate mixture in the prevention of γ-radiation-induced DNA damage in human lymphocytes as compared to NAC and cysteine. Methods: Tis study was carried out at the Iran University of Medical Sciences, Tehran, Iran, between April and September 2016. Citrated blood samples of 7 mL each were taken from 22 healthy subjects. Each sample was divided into 1 mL aliquots, with the frst aliquot acting as the control while the second was exposed to 2 Gy of γ-radiation at a dose rate of 102.7 cGy/minute. Te remaining aliquots were separately incubated with 600 μM concentrations each of serine, magnesium sulfate, serine-magnesium sulfate, NAC and cysteine before being exposed to 2 Gy of γ-radiation. Lymphocytes were isolated using a separation medium and methyl-thiazole-tetrazolium and comet assays were used to evaluate cell viability and DNA damage, respectively. Results: Te serine-magnesium sulfate mixture signifcantly increased lymphocyte viability and reduced DNA damage in comparison to serine, magnesium sulfate, NAC or cysteine alone (P <0.01 each). Conclusion: Te fndings of the present study support the use of a serine-magnesium sulfate mixture as a new, non-toxic, potent and efcient radioprotective agent.
Authors and Affiliations
Vahid Changizi| Department of Technology of Radiology & Radiotherapy, Allied Medical Sciences School, Mona Bahrami| Department of Technology of Radiology & Radiotherapy, Allied Medical Sciences School, Mahbod Esfahani| Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran, Seyed V. Shetab-Boushehri| Department of Toxicology & Pharmacology, International Campus and Razi Drug Research Center, Iran University of Medical Sciences, Tehran, Iran
Keywords
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- EP ID EP14768
- DOI 10.18295/squmj.2016.17.02.005
- Views 410
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