Proapoptotic activities of Oroxylum indicum leave extract in HeLa cells
Journal Title: Asian Pacific Journal of Tropical Biomedicine - Year 2019, Vol 9, Issue 8
Abstract
Objective: To examine the proapoptotic properties of Oroxylum indicum methanol extract on cervical cancer cells. Methods: Methylene blue assay was used to determine the IC50 value of the extract. Western blotting assays were done to analyze the expression of HPV oncoproteins (HPV18 E6 and E7) and apoptotic molecules (caspase-3 and caspase-8). Reverse transcriptase PCR assays were performed to determine genetic alteration of tumor suppressors p53 and pRb and apoptosis markers Fas and FasL. Enzyme-linked immunosorbent assay (ELISA) was done to determine the expression of cytokine levels (IL-6 and IL-12). Results: The determination of IC50 value indicated a higher anti-proliferative activity of the extract compared to cisplatin. After 24 hours of treatment, Western blot analysis showed that treated HeLa cells exhibited a significant down-regulation of HPV18 oncoproteins E6 and E7, and a significant induction of caspase-8 and caspase-3 activation level. Meanwhile, the mRNA expressions of p53, pRb, Fas and FasL were significantly upregulated in treated cells. Moreover, ELISA showed an increased IL-12 and decreased IL-6 production after Oroxylum indicum treatment. Conclusions: The methanol extract of Oroxylum indicum has an anti-proliferative activity and proapoptotic potential. It induces localized-immunity improvements by altering cytokine production in HPV-positive cervical cancer cells.
Authors and Affiliations
Nurul Hidayah Wahab, Nur Afina Mohd Din, Mohd Din, Noor Izani Noor Jamil, Nor Fazila Che Mat
Keywords
Related Articles
- EP ID EP617237
- DOI 10.4103/2221-1691.262080
- Views 65
- Downloads 0
Molecular study of HCV detection, genotypes and their routes of transmission in North West Frontier Province, Pakistan
Objective To determine hepatitis C virus (HCV) genotypes and explore the associated risk factors in chronic HCV patients. Methods A total of 116 patients with chronic hepatitis C were subjected to polymerase chain rea...
Chalcone analogue as potent anti-malarial compounds against Plasmodium falciparum: Synthesis, biological evaluation, and docking simulation study
Objective: To investigate in vitro antimalarial activity of chalcone derivative compounds against Plasmodium falciparum 3D7 (Pf3D7) strain and in silico antimalarial activity. Methods: Synthesis of the chalcone derivativ...
Phenolics, fatty acids composition and biological activities of various extracts and fractions of Malaysian Aaptos aaptos
Objective: To investigate phenolics, fatty acids composition and biological activities of various extracts and fractions of Malaysian Aaptos aaptos. Methods: Fatty acid methyl ester was analyzed by gas chromatography-fla...
Sapium ellipticum (Hochst) Pax ethanol leaf extract modulates glucokinase and glucose-6-phosphatase activities in streptozotocin induced diabetic rats
Objective: To examine the effects of Sapium ellipticum (SE) leaf extract on the hepatic activities of glucokinase and glucose-6-phosphatase in streptozotocin-induced diabetic Wistar rats. Methods: STZ-induced diabetic Wi...
Anti-quorum sensing and anti-biofilm formation activities of plant extracts from South Korea
Objective: To investigate anti-quorum sensing (anti-QS) and anti-biofilm formation (anti- BF) activities of the ethanol extracts of 388 plants. Methods: The anti-QS activity of the plant extracts was evaluated by disc-di...