Production and characterization of antimicrobials from isolate Pantoea agglomerans of Medicago sativa plant rhizosphere soil
Journal Title: Journal of Applied and Natural Science - Year 2019, Vol 11, Issue 2
Abstract
Due to rise in drug resistance among pathogens, there is always an urge to look for new drug alternatives. So in this study we aimed to identify the unexplored rhizosphere microflora of alfalfa plant for new antimicrobials. With initial screening for isolates from rhizosphere region for antibacterial activity against selected bacterial pathogens, the isolate AL10 had better activity selected for this study. The isolate mass was cultured and secondary metabolites were extracted using ethyl acetate and subjected to FTIR and GC-MS analysis. Based on functional diversity analysis, the isolate subjected to anti-bacterial activity revealed significant activity against Streptococcus pneumonia, Klebsiella, S. aureus with zone of inhibition in the range of18-20 mm. Based on GC-MS analysis report ten compounds were identified and 1-Octadecane and 1-nonadecanol were found to be responsible for bio-activity. FT-IR results showed that N-H stretching functional group was dominantly present in the extract. Molecular identification of the isolate by 16S rRNA sequencing showed the isolate as Pantoea agglomerans. The results showed that the isolate P.agglomerans, gram negative bacteria had wide antibacterial activity due to 1-Octadecane and 1-nonadecanol. Though Alfalfa plant has been described for various biological activities, this is a first report on rhizosphere region of plant reporting for antibacterial potential microbes.
Authors and Affiliations
Nisha M Nair, R. Kanthasamy, S. Iruthaya Kalai Selvam, S. Ramalakshmi
Keywords
Related Articles
- EP ID EP588655
- DOI 10.31018/jans.v11i2.2031
- Views 74
- Downloads 0
Comparative evaluation of water budgeting parameters under different rice (Oryza sativa L.) cultivation methods
Water budgeting studies under different rice cultivation methods provides an insight into the amount of water used by the plant and percolated below the root zone for judicious water management. To undertake this study,...
Rooting potential of mature stem cuttings of some forest tree species for vegetative propagation
The growing of trees could be hindered by seed dormancy problems or unavailability of sufficient quantities of seeds, hence vegetative propagation using simple propagules like stem cuttings is a valued option especially...
Screening a set of tomato parental lines and their hybrids for resistance to early blight (Alternaria solani) by detached leaf method
Nine parental lines and 18 hybrids derived by crossing them in Line × Tester fashion were screened against the early blight pathogen Alternaria solani along with a resistant, susceptible and a commercial check in the for...
Studies on development and storage stability of dehydrated pumpkin based instant soup mix
The study was carried out to develop and standardize Instant Soup Mix (ISM) from dehydrated pumpkin powder and to evaluate nutritional (moisture, sugars, protein, β-carotene, fat, fibre and water activity) and sensory qu...
Effects of different sub-lethal concentrations of plasticizer-Diethyl phthalate on Fresh water murrel, Channa striatus (Bloch)
In the present study, effects of exposure to different sub lethal concentrations of diethyl phthalate on hematological, biochemical and histological parameters of fresh water murrel, Channa striatus were evaluated. LC50...