Production and Characterization of Chitinases from Thermophilic Bacteria Isolated from Prataan Hot Spring, East Java
Journal Title: Journal of Mathematical and Fundamental Sciences - Year 2016, Vol 48, Issue 2
Abstract
Thermophilic bacteria producing chitinase were collected from Prataan hot spring, East Java, Indonesia and screened. The isolated bacterium was analyzed using 16S rRNA gene sequencing analysis and identified as Paenibacillus sp. The molecular identification was confirmed through morphological and physiological analyses. The production of chitinase was conducted at various incubation times, temperatures, pH and concentrations of colloidal chitin. The optimum condition of the isolate to produce the highest chitinase was 0.9% (w/v) of colloidal chitin (pH 7.0) at 48 °C for 24 hours. The obtained chitinases were optimally active at 55 °C and pH 6.0-7.0. The chitinases were gradually purified by ammonium sulfate precipitation, Sephadex G-100 gel filtration, followed by DEAE–cellulose ion exchange chromatography (IEC). The purification method gave a purification factor of 9.43 and a yield of 2.68%. Two protein fractions were obtained from sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with molecular weights of 68 and 82 kDa.
Authors and Affiliations
Ruth Chrisnasari, Steven Yasaputera, Pauline Christianto, Veronika Ivone Santoso, Tjandra Pantjajani
Keywords
Related Articles
- EP ID EP203690
- DOI 10.5614/j.math.fund.sci.2016.48.2.6
- Views 123
- Downloads 0
New Generalized Algorithm for Developing k-Step Higher Derivative Block Methods for Solving Higher Order Ordinary Differential Equations
This article presents a new generalized algorithm for developing k-step (m+1)th derivative block methods for solving mth order ordinary differential equations. This new algorithm utilizes the concept from the conventiona...
Inclusion Properties for a Class of Meromorphic Functions Defined by a Linear Operator
In the present paper, we study a certain class of meromorphic univalent functions f(z) dened by the linear operator L(α,β) f (z). The aim of the present paper is to prove some properties for the class Σα,β,kλ;(h) to sati...
Effect of V Dopant on Physicochemical Properties of Vanadium-Doped Anatase Synthesized via Simple Reflux Technique
Mesoporous pure TiO2 (M-TiO2) and mesoporous-vanadium-doped TiO2 (M-V-doped TiO2) were successfully synthesized via a facile and simple reflux technique. The purpose of this research was to study the effect of vanadium d...
Flavonoid Compounds from the Herb of Krokot (Lygodium microphyllum) and their antioxidant activity against DPPH
Six flavonoid compounds, kaempferol (1), quercetin (2), acacetin (3), quercetin-3-O-β-D-glucopyranoside (4), kaempferol-3-O-β-D-glucopyranoside (5), and isorhamnetin-3-O-β-D-glucopyranoside (6), were isolated from the me...
Robust Estimators for the Correlation Measure to Resist Outliers in Data
The objective of this research was to propose a composite correlation coefficient to estimate the rank correlation coefficient of two variables. A simulation study was conducted using 228 situations for a bivariate norma...