Production and Characterization of Cyanide Hydratase from Micromonospora braunna
Journal Title: Universal Journal of Environmental Research and Technology - Year 2012, Vol 2, Issue 6
Abstract
Cyanide hydratase [E.C. 4.2.1.66] is an intracellular enzyme detected in few fungi and bacteria. It catalyzes the reaction of hydration of cyanide, a potent toxin, to form formamide, a non-poisonous amide intermediate of cyanide degradation. When asked to name a poison, everybody nominates cyanide. This evil reputation of cyanide worth paying attention because of its increasing occurrence due to natural synthesis and also due to the large scale industrial production of inorganic cyanide as CN- and HCN as well as organic cyanides as nitriles, RCN. As cyanide is highly toxic, it must be detoxified in the effluent before discharging into the sewers. Although several processes of cyanide detoxification are available, the biological treatment of cyanide waste is cost-effective and ecofriendly. Cyanide hydratase is becoming important green catalyst for this process, which converts cyanide into formamide and ammonia without employing extreme conditions of reaction temperature and hydrogen ion concentration. The present project was undertaken with the view of approaching to the bioremediation of cyanide effluent. Potential cyanide degrader actinomycetes, identified as Micromonospora braunna was isolated and acclimatized in the minimal medium containing 1000 ppm cyanide. The detection of formamide in the culture broth confirmed the cyanide hydratase activity. 76.66 enzyme units were detected in the production broth supplemented with dextrose as carbon source and potassium cyanide as sole nitrogen source. The enzyme cyanide hydratase was extracted and its reaction parameters were determined. The kinetics studied revealed the Km value as 33mM and Vmax 39 µmoles/ml/min.
Authors and Affiliations
Shete H. G. and Kapdnis B. P.
Keywords
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