PRODUCTION OF BETALAINS FROM HAIRY ROOT CULTURE OF BETA VULGARIS AND ITS USE IN PARACETAMOL SYRUP AS A NATURAL COLOURANT
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2019, Vol 12, Issue 5
Abstract
Objective: The present study was aimed to extract betalains from hairy root culture of Beta vulgaris and its use in pharmaceutical formulations as a colorant. Methods: Hairy roots were initiated using different strains of Agrobacterium rhizogenes such as A.2/83, A.20/83, A.4, and LMG 150; LMG 150 was found to initiate a large number of hairy roots, and betalain content was estimated. Paracetamol syrup was prepared using extracted betalains as a colorant at different concentrations of 10 and 30 mg/150 ml. Stability studies were carried out at a different temperatures such as 25°C, 30°C, and 40°C) and light (dark, 1000 and 2000 lux) for 45 days. Results: In case of a concentration of 10 mg/150 ml syrup, the effects of temperature such as 25°C, 30°C, and 40°C and dark condition on the degradation of betalains were found to be 48%, 88%, and 100% in 45 days, respectively. The effects of temperature such as 25°C, 30°C, and 40°C at light 1000 lux on degradations of betalains were found to be 81% and 98% at 25°C and 30°C in 45 days, respectively, and 100% at 40°C in 30 days and at 2000 lux were found to be 100% at 25°C and 30°C in 30 days and 100% at 40°C in 10 days. The similar levels of degradation rate were observed with a concentration of 30 mg/150 ml. Conclusion: Experimental data demonstrated that formulation with betalains exhibited better stability at the dark condition and lower temperature.
Authors and Affiliations
ASHOKA BABU VL , MADHAVAN V
Keywords
Related Articles
- EP ID EP604769
- DOI -
- Views 135
- Downloads 0
FEBUXOSTAT MODULATES OXIDATIVE AND APOPTOTIC PATHWAYS IN ACUTE DOXORUBICIN‑INDUCED CARDIOTOXICITY: AN EXPERIMENTAL ANIMAL MODEL STUDY
Objectives: Doxorubicin is one of the most important and powerful anticancer drugs, the most pronounced limitation for its use is toxicity on normal cells. Mechanism of doxorubicin-induced cardiotoxicity (DIC) is multifa...
ERUCA SATIVA LINN.: PHARMACOGNOSTICAL AND PHARMACOLOGICAL PROPERTIES AND PHARMACEUTICAL PREPARATIONS
Eruca sativa (jarjeer) is an annual herb (family Brassicaceae), which contains a wide range of chemicals and minerals with nutraceutical and organoleptic characteristics. Jarjeer was generally used as a food and traditio...
COMPARISON BETWEEN DIETARY INTAKE AND AGE IN RELATION TO BODY MASS INDEX AMONG POLYCYSTIC OVARIAN SYNDROME WOMEN – A CASE–CONTROL STUDY
Objective: Polycystic ovarian syndrome (PCOS) is the most prevalent endocrine disorder, affecting up to 6.8% of women at their prime reproductive age. The aim of the study is to prove the interrelationship between food p...
NOVEL APPROACH OF EXTRACTION AND CHARACTERIZATION OF OKRA GUM AS A BINDER FOR TABLET FORMULATION
Objective: The major objective of the present investigation was to extract a natural polymer (okra gum) with its characterization as pharmaceutical binder and to formulate, develop, and evaluate the compression-coated ta...
EVALUATION OF ANTICONVULSANT ACTIVITY OF PREMNA HERBACEA (ROXB.) ROOT EXTRACTS IN ISONIAZID AND STRYCHNINE-INDUCED CONVULSIONS
Objective: The present investigation was to evaluate the anticonvulsant potential of three different extracts of roots of Premna herbacea (Roxb.) in mice. The preclinical screening models such as isoniazid (INH)- and str...