Abstract

Among the energy sources, the depletion of non-renewable energy is of great concern in the present days. The non- renewable energy deposits are continuously depleted by human withdrawals. An alarming situation of continuous depletion in the natural resources by an increased consumption of energy, the urge to look out for alternate ways for energy production in on the high stand. Anaerobic bacteria are known to be efficient energy producers. In the present study, the anaerobic bacterial hydrogen production from different consortia isolated from different sources was studied. Anaerobic consortia were isolated from different sources by inoculating into specific media, Beibl and Pfennigs medium anaerobically in the light (2000 lux). Bergey’s Manual of Systematic Bacteriology (1994) was adopted for identification of phototrophic bacteria. Growth was determined using UV-Vis spectrophotometer at 660nm. The effect of two major substrates i.e. Benzoate and Cellulose has been investigated. The amount of biogas produced varied with benzoate and cellulose as substrates. Among the 10(ten) consortia, for most of the consortia cellulose was confirmed to be utilized as a major substrate for the production of biogas.

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