Production of extracellular proteases by Aeromonas, Serratia and Shewanella species and characterization of extracellular proteases using zymogram analysis.
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 5
Abstract
Seafood spoilage microorganisms are known to produce extracellular proteases. The current research was aimed at using SDS-PAGE substrate impregnated gel to characterize the proteases secreted by the spoilage bacteria. Extracellular proteases (ECP) of seven spoilage microorganisms namely; Shewanella baltica OS185, Aeromonas sp. HB-6,, A. hydrophila HX 201006-3, Sh. baltica, Sh. baltica OS678, Serratia spp. I-113- 31 and A. salmonicida subsp. achromogenes isolated from various seafoods were analyzed by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) using Coomassie brillant blue-stain. Extracellular proteases of the seven spoilage microorganisms were characterized by substrate (gelatin and sheep blood) co-polymerized SDS-PAGE. The proteolytic activity results of the (ECPs) the spoilage microoganisms revealed the presence of many bands ranging in molecular weight from 20 to 100 kDa which represents serine, cysteine, aspartic and metallo proteases. The gelatinase profiles for Aeromonas spp., Shewanella spp. and Serratia spp. were characterized by the presence of high molecular weight metallo proteases ranging from 69, 70, 100, 110,140 and 220 kDa; 70, 100 and 120 kDa and 100 kDa, respectively. Whereas the sheep-blood profiles for Aeromonas spp., Shewanella spp. and Serratia spp. were characterized by the presence of serine, cystein, aspartic and high molecular weight metallo proteases ranging from 37, 50 and 100 kDa; 35, 37, 50 and 100 kDa and 44 kDa respectively.
Authors and Affiliations
Ngozi Izuchukwu
Keywords
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- EP ID EP392076
- DOI 10.9790/3008-1205074249
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