Production of Fat and Sterol by Aspergillus nidulans in Submerged Fermentation
Journal Title: Chemical Science International Journal - Year 2017, Vol 20, Issue 2
Abstract
The efficiency of fat and sterol production by Aspergillus nidulans was evaluated. The fungus was grown in media containing various carbon and nitrogen sources. Some agricultural waste materials namely, corn cob, rice husk and potato peels were also used as sole carbon sources for fat and sterol production. Sodium acetate was added into culture media at various concentrations. After a 72 h incubation, mycelia obtained from culture media were assayed for fat and sterol contents. Among the sole carbon sources used for fat production, the highest yield of 48.63 mg/100 mL broth was achieved with mannitol after 72 h incubation. Carboxyl methyl cellulose was the poorest carbohydrate for fat production. The agricultural waste materials greatly improved fat production by Aspergillus nidulans. Levels of 44.62, 43.99 and 43.1 mg/100 mL broth were achieved with corn cob, rice husk and potato peels after 72 h incubation. Mannitol also caused the best sterol production by the fungus. Monosodium glutamate and corn steep liquor were the best nitrogen sources for the production of fat and caused the production of 64.36 and 64.27 mg/100 mL broth respectively. Urea and potassium nitrate did not support good fat production by the fungus. Hydrolyzed potato peels supported high yields of sterol as compared to mannitol. Increased fat production was accomplished through the addition of 0.1 to 0.5% sodium acetate. Maximum fat yield of 168.67 mg/100 mL broth occurred after 72 h in medium in which acetate concentration was 0.4%. This level was significantly higher than that of the control which had only 34.82 mg/100 mL broth of fat after 72 h. Acetate addition also caused reasonable increases in sterol synthesis higher than the control.
Authors and Affiliations
Ogbonnaya Nwokoro, Ogechi H. Ekwem, Uchenna Oyeagu
Keywords
Related Articles
- EP ID EP319619
- DOI 10.9734/CSJI/2017/35197
- Views 89
- Downloads 0
Exploring Gmelina arborea Leaves for Biofuels and Petrochemical and Pharmaceutical Feedstocks
An investigation was carried on the chemical constituents of Gmelina arborea leaves by hydrolyzing the pulverized dry leaves with 3% sulphuric acid solution at 100°C for 30 minutes. The extract on analysis with GCMS yiel...
Production of Mucuna pruriens (var. utilis) Proteins Isolates Using Central Composite Design and Effect of Drying Techniques on Some Properties
Response surface methodology was used to model the effect of temperature, NaCl concentration and pH on the production of protein from Mucuna pruriens seeds. The analysis of response surface graphs showed that the optimal...
Preparation, Microstructure and Performance of Activated FA / TiO2-WO3 Composite Photocatalyst
The goal of this article was to preparation Fly ash(FA)/TiO2-WO3 Composite Photocatalyst based activation and modify of FA surface using simple chemical method. The FA carrier and catalysts were investigated through XRD...
Isolation and Characterisation of an Αlpha-amyrin Acetate Isolated from the Leaves of Microtrichia perotitii DC (Asteraceae)
Aims: Microtrichia perotitii DC belongs to the family Asteraceae and is widely distributed in West African countries of Nigeria, Senegal, Mali, Port of Guinea, Sieraleone, Ivory Coast, Ghana and Dahomey. In Nigeria it is...
Synthesis and Intrinsic Viscosity-molecular Weight Relationship of Poly(ethylene adipate)
In this work, two series of poly (ethylene adipate) (PEA) samples with broad and narrow molecular weight distribution (MWD) were prepared by the polymerization between adipic acid and ethylene glycol. An accurate and swi...