Production of Lutein-enriched Biomass by Growing Coccomyxa sp. (strain onubensis; Chlorophyta) under Spring Outdoor Conditions of Southwest Spain
Journal Title: JOURNAL OF ADVANCES IN BIOTECHNOLOGY - Year 2016, Vol 6, Issue 1
Abstract
Coccomyxa sp. (strain onubensis) is an acidic environment microalga, which was grown under spring outdoor conditions of Huelva province (southwest, Spain). A 6 L pilot tubular photobioreactor was used to determine the influence of the environmental light and temperature conditions on the microalga growth and its intracellular content of carotenoids. Cloudy days supplied the adequate light intensity (maximum 1070 μmol photons m-2 s-1) and temperature (maximum 32ºC) regimes for a biomass productivity of 0.28 g L-1 d-1 with an intracellular content of lutein (4.8 mg g-1 dry weight) and eventually β-carotene (0.8 mg g-1) obtained at the end of the day evening. This work supplies guidelines for a large-scale outdoor culture of Coccomyxa. sp. to supply lutein-enriched biomass, with the advantage of the growth at pH 2.5, which preserves cultures from contamination.
Authors and Affiliations
Isabel Vaquero, M. Carmen Ruiz-Domínguez, Benito Mogedas, Carlos Vílchez, José M. Vega
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