Production of Xylanase by Aspergillus flavus FPDN1 on Pearl millet bran: Optimization of Culture Conditions and Application in Bioethanol Production
Journal Title: International Journal of Research in Chemistry and Environment (IJRCE) - Year 2012, Vol 2, Issue 3
Abstract
The production of extracellular xylanase by a locally isolated strain of Aspergillus flavus FPDN1 was studied for optimization of cultural conditions and saccharification followed by bioethanol production. Among the various agro residues used pearl millet (bajara) bran was found to be the best for high activity of xylanase with poor cellulase production under solid state fermentation with compared to submerged fermentation. During optimization, influence of different cultural conditions such as substrate concentration, incubation period, moisture level, inoculum size, pH, temperature, effect of carbon sources, orgenic and inorganic nitrogen sources were investigated. The production of the xylanase reached at maximum with substrate concentration (4g), incubation period (5 day), moisture level (45%), inoculum size (3 x 106 spores/mL), pH (6), temperature (250C), carbon source (xylose), organic nitrogen source (yeast extract) and inorganic nitrogen source [(NH4)2SO4]. Under optimized conditions enhanced xylanase production achieved was 1530 IU/gds/min. The hydrolysis study of birch wood xylan by TLC was yielded xylose and other xylooligosaccharides as an end product. Crude xylanase was further utilized for enzymatic saccharification of pearl millet bran followed by bioethanol production from saccharified sugars. It was further confirmed by GLC analysis.
Authors and Affiliations
Bhatt Nikhi, Adhyaru Dharmesh, Thakor Priti
Keywords
Related Articles
- EP ID EP104285
- DOI -
- Views 111
- Downloads 0
[b] Change in Geotechnical Properties of High Plastic Soils on Exposure to Acid Rains of Varying Strength[/b]
[i] Acidification of rain caused by air pollutants like sulfur dioxide and oxides of nitrogen etc. reduces the pH as low as 3 to 4. The low H+ concentration of acid rain falling for a short duration may not affect s...
[b]Adsorption of Metal (Cd) from Synthetic Wastewater by Plant Material [/b][b][/b]
[b][i]The major objective of this paper was to investigate the removal of Cadmium (II) from synthetic wastewater using poly vinyl alcohol coated activated carbon prepared from leaves of Calotropis Procera (PVAC-...
Synergistic Extraction of Uranium (VI) by Complexation with CYANEX-272 and CYANEX-923, TPBD, TNBD, TOPO in Presence of Nitrate
Synergistic extraction of uranium (VI) from nitrate medium by cyanex 272 (HA)2 along with cyanex 923, TPBD, TNBD, TOPO and their mixtures in xylene as extractants has been investigated. Analysis of the extraction data by...
Physico-Chemical Studies of Binary Mixture of Triethylene Glycol Monomethyl Ether and Water at T= (298.15, 308.15, 318.15, 328.15) K
The densities and viscosities η have been measured as a function of composition for the binary mixtures of triethylene glycol monomethyl ether with water at (298.15,308.15,318.15, 328.15)K From the experimental data,...
Kinetics and Hydrolysis Mechanism of 2,4-dichlorofenoxyacetic acid
The hydrolytic transformation kinetics of the 2,4-dichlorofenoxyacetic acid (2,4-D) was investigated as a function of pH. The hydrolysis rate was pH-dependent and the first order. The hydrolysis was much faster in acidic...