Profil Hematologi Tikus (Rattus norvegicus Berkenhout, 1769) Galur Wistar Jantan dan Betina Umur 4, 6, dan 8 Minggu
Journal Title: Biogenesis: Jurnal Ilmiah Biologi - Year 2014, Vol 2, Issue 2
Abstract
Animal model is very important in biomedical research. Prior to apply in human or other primates, experiments using lower animals model such as rodents must be conducted (preclicical studies). Wistar rats (Rattus norvegicus Berkenhout, 1769) are used extensively for in vivo studies, particularly in physiology. However, their normal hematology profile for baseline or control has not been yet available locally. Many researchers adopt data from general references such as clinical or laboratory manual books, journals, textbooks, or other resources, which definitely affect the validity of their results. This study was carried out to provide normal hematology profile of male and female Wistar rats bred in animal house of Laboratorium Penelitian dan Pengujian Terpadu Universitas Gadjah Mada (LPPT-UGM). Subjects were four-, six-, and eight-week-old rats, representative for juvenile/ immature, preadult, and adult/mature, respectively. Procedures following routine standard method in hematology: blood withdrawal from sinus retro-orbitalis of ketamine-anesthetized animals and analysis using Hematology Analyzer. Variables were number of erythrocytes, hemoglobin, hematocrit, number of leukocytes and differential count, number of thrombocytes, and body mass. Results indicated that hematology profile and body mass of rats from different sex and different age were varied. It is fundamental to have local baseline data, which directly come from animal house. Adoption from other references should be avoid due to generality or unspecified animal, lack of information on methods, and some works have already outdated. Different sex, age, environmental factors, and method affect physiological condition in animals, result in different hematology profile.
Authors and Affiliations
Laksmindra Fitria, Mulyati Sarto
Keywords
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- EP ID EP294514
- DOI 10.24252/bio.v2i2.473
- Views 186
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