Propagation of infectious pancreatic necrosis virus in fish cell cultures
Journal Title: Біологія тварин - Year 2014, Vol 16, Issue 4
Abstract
The reproduction of the Ukrainian isolate of infectious pancreatic necrosis virus (IPNV) “Carpathians” in fish continuous cell cultures of RTG-2, FHM and EPC was investigated. All three cell lines were sensitive to virus. IPNV caused morphological changes, such as vacuole enlargements and cells rounding. Subsequently cells scaled from a surface and characteristic cytopathic effect (CPE) of virus on cells was visible. For cell lines of RTG-2 and FHM the complete destruction of monolayer was noted on 7–8 day after infection (d.a.i.). For culture of EPC characteristic CPE and complete destruction of cell monolayer were marked on 10-12 d.a.i. Infectious titer of IPNV “Carpathians” in studied cell lines was following for EPC 105,5–5,8 TCID50/ml, and for the lines of FHM and RTG-2 — 106,2–6,5 and 106,9–7,4 TCID50/ml respectively. The low infectious titer of virus in cells of EPC can be related to its slow reproduction in this culture. The greatest infectious titer was observed for the culture of RTG-2, that is fully appropriately, as this cell line was derived from a rainbow trout — natural IPNV reservoir. That is why the RTG-2 is the most appropriate cell lines for accumulation of the Ukrainian isolate IPNV “Carpathians”. But for diagnostics of the Ukrainian isolate IPNV “Carpathians” all three cell cultures of RTG-2, FHM and EPC can be used. Results of our electronic-microscopy researches of purified viral particles revealed basic for birnaviruses morphology and ultrastructure characteristics. Virions of the Ukrainian isolate of IPNV “Carpathians” had a hexagonal form, their diameter was 70±5 nm. For subsequent research of isolate “Carpathians” the methods of molecular diagnostics are needed, which will allow to determine the genotype of this IPNV strain and his possible origin.
Authors and Affiliations
M. Maistrenko, Yu. Rud, L. Buchatsky
Polymorphism of microsatellite DNA sequences in dogs Russian Toy Terrier and German Dog
The level of genetic polymorphism of five micro-satellite loci for the German Dog and the Russian Toy-Terrier dog breeds which are bred in Ukraine is researched. The study is aimed to value the micro-satellite panel for...
Physical parameters of cervical mucus and its correlation with stimulated and non-stimulated ewe fertility
The indexes of stretchability, electric resistance and crystallization of cervical mucus, pumped out in vaginal part of cervix, and its correlation with fertility were investigated on 65 Ascanian Merino ewes. Mucus was o...
Chelators, their properties and applications
The analysis of summarized data about the nature of the interaction of enterosorbents with functional elements of cells and the products of its metabolism is presented in the paper. The system of notions about biology of...
Chemical composition and nutritional values of feed resources for deer
Increasing of wild animals populations and saving of endangered species require proper providing of nutrient needs. In general, the available feed resources provide the feed needs of wild herbivores, but the ratio of the...
Antibacterial activity of complex of enrofloxacin with nanopolymer GluLa-DPG-PEG600
This article presents the results of the study of antibacterial activity of complex of enrofloxacin with nanopolymer based on pseudo-polyamino acids GluLa-DPG-PEG600. Nanopolymer GluLa-DPG-PEG600 has low toxicity to mamm...