Protective Effect of N-Acetylcystein and Resveratrol on Ischemia-Reperfusion Injury in Rat Ovary

Apply

Protective Effect of N-Acetylcystein and Resveratrol on Ischemia-Reperfusion Injury in Rat Ovary

Journal Title: Dicle Tıp Dergisi - Year 2016, Vol 43, Issue 2

Abstract

Objective: The aim of this study is evaluating the protective activity of N-acetyl cysteine and resveratrol treatment against ischemia - reperfusion damage created experimentally in rat ovaries. Methods: 42 female Wistar rats were used in our study. Rats were separated randomly into six groups consisting of seven rats as sham, torsion, torsion- detorsion, torsion-detorsion+saline, torsion-detorsion+resveretrol (20 mg/kg) and torsion- detorsion+N-acetyl cysteine (150 mg/kg). Except Sham, ovarian torsion procedure was implemented to all other groups for 2 hours. Detorsion procedure was implemented to other groups for 2 hours, except the torsion group. Medications were given through intraperitoneal way half an hour before the detorsion procedure in saline (two milliliter), resveratrol (20 mg/kg) and N-acetyl cysteine (150 mg/kg) groups. Then, 2 ml of blood samples were drawn for markers of oxidative stress and tumour necrosis factor-alpha (TNF-α) work and the ovaries, which were torsioned for the histologic examination, were extracted from all rats. Edema, congestion, hemorrhage, leukocyte infiltration and degeneration of follicles were evaluated by histopathological examination. Results: According to histopathologic damage scores, the least damage was seen in sham group and the most damage was seen T-DT group (1.00±0.81 vs. 11.00±1.15, respectively; p<0.001). It was seen that resveratrol and N-acetyl cysteine treatments were effective in decreasing tissue damage (total damage score average 83.85±0.89 vs. 3.85±0.89, respectively; p<0.001), and on the other hand there was not any difference between resveratrol and N-acetyl cysteine treatments (p=0.966). Besides, it was determined that oxidative stress levels were higher in torsion - detorsion group and the resveratrol and N-acetyl cysteine treatment caused a significant decrease in oxidative stress levels. In additionally, the reductions of TNF-α levels were found to be equally effective in both drugs (8.68±1.88 vs. 7.85±2.08, P=0.968). Conclusion: Presented study showed that resveratrol and N-acetyl cysteine treatment can be effective in preventing tissue damage and oxidative stress, which is induced by ischemia-reperfusion that is created in rat ovaries. On the other hand, no difference was found between the resveratrol and N-acetyl cysteine with regards to protective activity. Key words: ischemia-reperfusion, ovary, N-acetylcysteine, resveratrol

Authors and Affiliations

Avni Kılıç, Hacer Uyanıkoğlu , Adnan İncebıyık

Keywords

Acute cholecystitis in pregnancy: Retrospective evaluation of 21 patients

Objective: We aimed to evaluate our diagnosis and treatment approach to pregnant patients with acute cholecystitis in our high birth rate region. Methods: Twenty-one patients who suffering from acute cholecystitis duri...

Distribution of community-acquired gram negative microorganisms detected in urine samples of pediatric patients and antibiotic resistance patterns at 2013 

Objective: This study aims to determine the antibiotic susceptibility and distribution of community-acquired microorganisms obtained from urine samples of patients admitted to our clinic in Diyarbakır, and also detect th...

EP ID EP133518
DOI 10.5798/diclemedj.0921.2016.02.0672
Views 90
Downloads 0