Protective effect of vitamin E on oxidative stress and sperm apoptosis in diabetic Mice
Journal Title: International Journal of Reproductive BioMedicine - Year 2019, Vol 17, Issue 2
Abstract
Background: Generation of free radicals and oxidative stress are a major contributor to diabetes. These factors lead to the development of diabetic testicles disorders. Objective: In this study, the protective effect of vitamin E on functional disorders associated with diabetes induced oxidative stress in male reproductive systems has been investigated. Materials and Methods: Thirty-three adult male Mice were divided into control, diabetic, and untreated diabetic groups. Streptozotocin was used to induce diabetes. In the treated group, vitamin E was given to the Mice intraperitoneally for 30 days. Then, animals were anesthetized and sacrificed. Animal testicles were isolated and homogenized in phosphate buffer and used for measuring sperm count, motility and survival of sperm, MDA concentration and antioxidant capacity (TAC). Apoptosis was also performed with the TUNEL test. Results: The results of reduction (12.03±98.11) TAC, MDA concentration (–28.5±2.58), sperm motility (unstable sperma= 86.4±7.48), sperm count (171.51), Sperm morphology (natural morphology= 49.69±31.93) and abnormal morphology (9.77±49.7) with increased oxidative damage. These changes were statistically significant in comparison with the control group for all variables other than MDA (p= 0.05). Treatment of vitamin E diabetic Mice improved the ability of antioxidants to prevent oxidative damage in the testicles, restore the sperm movement, and increase the number of normal sperm as well as TAC. The level of apoptosis in the treated group has decreased compared to the untreated group. Conclusion: Vitamin E protects the reproductive system against diabetes mellitus. Therefore, it was concluded that vitamin E may be a suitable agent for protecting the sperm and testicular parameters against undesirable effects of diabetes.
Authors and Affiliations
Khadijeh Mirzaei Khorramabadi, Ali Reza Radkhah, Abolghasem Abbasi, Aghdas Mirjalili
Keywords
Related Articles
- EP ID EP642586
- DOI -
- Views 77
- Downloads 0
Urogenital chlamydia trachomatis treatment failure with azithromycin: A meta-analysis
Background: Chlamydia Trachomatis is one of the most common pathogens transmitted through the genital tract in humans that leads to urogenital infection. Objective: Given the high prevalence of chlamydia infection and it...
Evaluation of the effect of follicular stimulating hormone on the in vitro bovine spermatogonial stem cells self-renewal: An experimental study
Background: Spermatogonial stem cells (SSCs) are undifferentiated cells which are highly reproducible and expandable. Several studies have been conducted to reproduce these cells in culture. They used growth factors, hor...
Self-compassion training and psychological well-being of infertile female
Background: The empowerment of psychological well-being is an important and fundamental issue among infertile females. Objective: The present study investigates the effect of teaching self-compassion on the psychological...
The effects of Salvia officinalis L. on granulosa cells and in vitro maturation of oocytes in mice
Background: Salvia officinalis L. has been used since ancient times but there are little data about effects of this herb on normal reproductive cells. Objective: To investigate the toxicity effects of Salvia officinalis...
Investigating the effects of Citrullus colocynthis pulp on oxidative stress in testes and epididymis in streptozotocin-induced diabetic male rats
Background: Diabetes mellitus is one of the most common metabolic diseases in humans, affecting 100 million people around the world. Objective: Investigating the effects of Citrullus colocynthis pulp on oxidant and antio...