Protective Effects of Mouse Bone Marrow Mesenchymal Stem Cell Soup on Staurosporine Induced Cell Death in PC12 and U87 Cell Lines
Journal Title: International Journal of Medical Research & Health Sciences (IJMRHS) - Year 2016, Vol 5, Issue 11
Abstract
Mouse bone marrow mesenchymal stem cells (mBMSCs) soup is promising tool for the treatment of neurodegenerative diseases. mBMSCs soup is easily obtained and is capable of transplantation without rejection. We investigated the effects of mBMSC soup on staurosporine-induced cell death in PC12 and U87 cells lines. The percentage of cell viability, cell death, NO concentration, total neurite length (TNL) and fraction of cell differentiation (f%) were assessed. Viability assay showed that mBM soup (24 and 48h) in time dependent were increased cell viability (p<0.05) and also cell death assay showed that cell death in time dependent were decreased, respectively (p<0.05). TNL and fraction of cell differentiation significantly were increased compared with treatment1 (p<0.05). Our data showed that mBM Soup protects cells, increases cell viability, suppresses cell death and improvement the neurite elongation. We concluded that Mouse bone marrow mesenchymal stem cell soup plays an important protective role in staurosporine-induced cell death in PC12 and U87 cell lines.
Authors and Affiliations
Hossein Zhaleh| Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran, Mehri Azadbakht| Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran, Ali Bidmeshki Pour| Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran Corresponding Email: a.bidmeshkipour@gmail.com
Keywords
Related Articles
- EP ID EP12211
- DOI -
- Views 272
- Downloads 12
Te Management Strategies used for Conflicts Resolution: A Study on the Chief Physician and the Directors of Health Care Services
Background: This study was performed using a descriptive concept to state reasons for conflict viewed from the perspective of head physicians and health care services directors who work within hospitals. Aims: This stu...
CYSTIC SWELLINGS OF SCROTUM: MANAGEMENT
The cystic swellings of scrotum are one among the commonest clinical entities faced by a surgeon in day to day practice and a cause of concern to the patient more with his fertility. Presenting with varied etiology they...
Care experiences and challenges of inpatients' companions in Iran's health care context: A qualitative study
Among the clients of nursing services, family members, as patient companions, serve as both caregiver and care receiver, such that specialized care is delivered by nurses and primary care is delegated to the patient com...
Human Papilloma Virus 16 and 18 Association in Cervical Intraepithelial Lesions and Cervical Cancers by In Situ Hybridization
Objective: To correlate the association of high risk Human Papilloma Virus (HPV) 16, 18 in cervical intraepithelial lesions and cervical cancers by in-situ hybridization (ISH) technique. Study Group: Cervical biopsy and...
THE EFFECT OF DIFFERENT BOUTS OF EXERCISE ON WEIGHT LOSS IN OVERWEIGHT INDIAN FEMALE UNDERGRADUATES
Background: The prevalence of obesity is increasing globally and in India with lack of sufficient time for continuous exercise being one of the causes. Performing multiple short bouts of exercise has been introduced as...