Protective Effects of Omega Supplement on Induced Hepatic Mal-Function by Thioacetamide in Male Rats
Journal Title: Zahedan Journal of Research in Medical Sciences - Year 2017, Vol 19, Issue 3
Abstract
Background: Exposure to thioacetamide causes hepatotoxicity and hepatocellular carcinoma in human, while fish oil omega-3 supplement has anti-inflammatory effects. Objectives: In this study, the protective effect of fish oil omega-3 supplement against induced hepatic mal-function by thioacetamide in rat is investigated. Methods: In this experimental study, 42 male rats were divided into 6 groups of seven. The control group, the sham 1 group receiving 0.4 mL/kg olive oil as the solvent of fish oil omega-3 supplement, the sham 2 group inter peritonealy receiving a dose of 150 mg/kg thioacetamide at the end of the experiment, the experimental groups 1, 2 and 3 orally receiving a daily dose of 100, 200 and 300 mg/kg fish oil omega-3 supplements respectively for 3 month followed by an inter peritoneal dose of 150 mg/kg thioacetamide at the end of the experiment. The serum levels of SGOT, SGPT, ALP, GGT, LDH, albumin, bilirubin and total protein were measured. Following hematoxylin-eosin staining, liver tissue samples were pathologically studied. The data were analyzed by SPSS-18 program and using one way ANOVA and Tukey as post hoc test. Results: The mean concentration of SGPT and bilirubin showed a significant decrease in the experimental group 1 compared to the group receiving thioacetamide. Similarly, the mean level of Albumin showed a significant reduction in experimental groups 1, 2 and 3 receiving thioacetamide. Also, the mean concentration of GGT in the experimental groups 1 and 2 compared to the group receiving thioacetamide decreased significantly; whereas, the mean levels of LDH, ALP, SGOT and total protein showed no significant changes in the experimental groups 1, 2, and 3. Finally, the tissue changes in all three experimental groups improved compared to the group receiving thioacetamide, and these effects were dose-dependent (P < 0.05). Conclusions: The results of this study indicate that fish oil omega-3 supplement has a protective effect on induced hepatic mal-function by thioacetamide in rat.
Authors and Affiliations
Davood Moghadamnia, Mokhtar Mokhtari, Saeed Khatamsaz
The Effectiveness of Dialectical Behavior Therapy in Decreasing High Risk Behaviors Among Students Suffering From Attention Deficit / Hyperactivity Disorder
Background: Attention deficit/hyperactivity disorder is a kind of disorder that may lead to interpersonal, emotional, educational and domestic problems. Moreover, it may lead to high-risk behaviors among teenagers and th...
The effect of two nutritional methods in changes of anthropometric indexes in obese parents and their children
Background: Parents obesity has a direct relation with their children obesity. The aim of this study is to determine the effect of two methods of weight loss in obese parents and their children. Materials and Method: W...
Frequency and Clinical Manifestations of Scabies in Suspected Patients Referred to Health Centers of Kashan, Central Iran (2010 - 2014)
Background: Scabies is one of the most common itching contagious skin disorder in the world. The agent of disease is Sarcoptes scabiei. Objectives: This study was conducted to determine the frequency and clinical manife...
Comparative Investigation of Mental Health Status of Spouses of War Handicaps in Accordance with Husband's Disability
Background: In connection with specific situation of handicaps of war in terms of both mental and physical health, their wives are dealing with peculiar stresses. In this research the mental health status of war victims...
CTX-M-15 Type β-lactamases From Clinical Isolates of Escherichia coli by Polymerase Chain Reaction and DNA Sequencing
Background: E. coli is a common cause of urinary tract infection and bacteremia in humans. That is frequently resistant to β-lactam antibiotics. Objectives: The aim of this study was to determine the antibiotic resistan...