Protective role of prostaglandin E1 analog in indomethacin-induced deterioration in acute respiratory distress syndrome in rats

Journal Title: National Journal of Physiology, Pharmacy and Pharmacology - Year 2017, Vol 7, Issue 7

Abstract

Background: Acute respiratory distress syndrome (ARDS) is a severe inflammatory condition. Our earlier studies have characterized oleic acid (OA)-induced rat model of ARDS which was exacerbated by indomethacin (prostaglandin [PG] synthesis inhibitor). Aim and Objectives: The role of PGs in ARDS is ill defined as the results of earlier studies are conflicting. This study was undertaken to determine the effect of PGE1 analog (misoprostol) in indomethacin-induced exacerbation of ARDS in rats. Materials and Methods: The rats were anesthetized with urethane. Tracheal and jugular vein cannulation was done to keep the respiratory tract patent and deliver drugs, respectively. Respiratory excursions were recorded with the help of force displacement transducer. Cannulated carotid artery was connected to pressure transducer for recording of blood pressure. Electrocardiographic potentials were recorded by needle electrodes. Animals were divided into four groups. In Group I, OA (75 µl) was used to induce ARDS in rats. In Group II, OA was injected in indomethacinpretreated rats. In Group III (control group), animals were treated with ethanol. In Group IV, OA was administered after indomethacin + misoprostol pretreatment. Misoprostol treatment was repeated after OA injection at 20 min interval. Cardiorespiratory parameters (respiratory frequency, heart rate, mean arterial pressure, and pulmonary water content) were determined, and histological examination of the lung was done in all groups. Results: Indomethacin pretreatment drastically advanced the OA-induced ARDS. Misoprostol protected against the deterioration as indicated by improvement in all the parameters and increase in survival time. Conclusion: Results of this study indicate that PGs have protective role in ARDS.

Authors and Affiliations

Parul Sharma, Ratna Pandey, Shripad B Deshpande

Keywords

Related Articles

Dapsone-induced methemoglobinemia in immune thrombocytopenia: A case report

ITP is a bleeding disorder which is not associated by a systemic disease caused by low platelet count or thrombocytes. However, use of dapsone is limited by adverse effects such as methemoglobinemia, reticulocyte increas...

In vitro antifungal effect of Rosmarinus officinalis essential oil on Aspergillus niger

Background: The development of Aspergillus niger fungi at the surface and in the food products is indeed very often seen, especially in the tins. It is essential to find a solution for a better conservation using natural...

Lipid profile, Thyroid profile and Eating behavior in Pre-hypertensive women

Background: Hypertension is a common health problem in developed countries. Individuals with systolic blood pressure (BP) between 120 and 139 mmHg or diastolic BP between 80 and 89 mmHg are categorized as prehypertensive...

 New hypothesis for mechanism of sliding filament theory of skeletal muscle contraction

 Background: Current understanding of skeletal muscle contraction is based on the sliding filament theory proposed independently by A.F. Huxley and H.E. Huxley (1954). The sliding filament theory very well explaine...

Comparative assessment of stress among medical students in relation to ABO blood groups

Background: Stress is a term that refers to the sum of physical, mental, and emotional strains or tensions on a person. Medical education can impose significant psychological stress on undergraduates. Considerable degree...

Download PDF file
  • EP ID EP290107
  • DOI 10.5455/njppp.2017.7.0305924032017
  • Views 74
  • Downloads 0

How To Cite

Parul Sharma, Ratna Pandey, Shripad B Deshpande (2017). Protective role of prostaglandin E1 analog in indomethacin-induced deterioration in acute respiratory distress syndrome in rats. National Journal of Physiology, Pharmacy and Pharmacology, 7(7), 749-753. https://europub.co.uk/articles/-A-290107