Protein Confinement in the Anodic Porous Alumina Microarray
Journal Title: NanoWorld Journal - Year 2017, Vol 3, Issue 0
Abstract
Alternative approaches to solve the complexity of protein function in the proteomic field required new arrays in nano-micro-scale dimension. Anodic Porous Alumina (APA), a geometrically ordered inorganic oxide, grown from ultrapure aluminum by anodic oxidation, was used in this work as an ordered array for the confinement of protein molecules by spin-coating technique. This microor nanostructured material can be used in different ways, both for the traditional fluorescence approach and for novel label-free methods. The aim of the present study is the proof of principle that the protein molecules can enter the APA micropores, as shown by fluorescein-labeled lysozyme confined into the APA template. Fluorescence intensity measurement as well as Raman Spectroscopy confirm that protein was incorporated into the APA micropores.
Authors and Affiliations
Claudio Larosa, Stefano Fiordoro, Eugenia Pechkova
Keywords
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- EP ID EP311565
- DOI 10.17756/nwj.2018-S1-005
- Views 97
- Downloads 0
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