Proteomic Analysis of Natural and Demucilaged Coffee Beans from Plantations at Different Altitudes in the Mantiqueira Mountains
Journal Title: Journal of Experimental Agriculture International - Year 2017, Vol 19, Issue 4
Abstract
Analysis of the effects of climatic conditions and processing methods on coffee beans is of great interest in research, chemical composition and consequent beverage quality are strongly influenced by growing conditions and/or processing methods. Aims: In this study, beans of Coffea arabica cv. Acaiá coffee trees grown at two altitudes (below 1000 m and above 1200 m) were assessed through a proteomic approach in an attempt to better understand the protein changes that occur during two types of processing (dry and wet processing). Methodology: Samples of proteins of natural and demucilaged coffee bean were analyzed by two-dimensional electrophoresis. The gels stained with Commassie Blue G250 were evaluated by the ImageMaster 2D Platinum 7.0 program. The differentially abundant proteins were treated with the enzyme Trypsin e aliquots of each hydrolysate sample were analyzed by mass spectrometry. Sample molecular mass profile obtained through MS e MS/MS was subjected to comparative analysis using the MASCOT program, Brazilian Coffee Genome database, NCBI and Coffee Genome Hub. Place and Duration of Study: The experiments were conducted in the following laboratories: the Laboratório Central de Biologia Molecular (LCBM) and the laboratório de Processamento de Produtos Agricolas (LPPA) at Federal University of Lavras, (Brazil), between 2014 and 2017. Results: Differentially accumulated proteins, such as 11S globulin, glyceraldehyde-3-phosphate dehydrogenase, dehydrin, and Heat shock proteins, have been found among treatments through mass spectrometry. Conclusion: These results contribute to an understanding of the effects of altitude and processing methods on the chemical composition of coffee beans, and thus serve as a basis for future research related to coffee beverage quality.
Authors and Affiliations
Kalynka G. do Livramento, Flávio M. Borém, Luana F. Torres, Fabrício L. da Silva, Dárlan Einstein do Livramento, Luciano V. Paiva
Keywords
Related Articles
- EP ID EP315477
- DOI 10.9734/JEAI/2017/38343
- Views 91
- Downloads 0
Effect of Boron and Putrescene on Russet Asian pear (Pyrus spp. L.) under Subtropical Condition of Jharkhand Province of India
A russet or brown spot in Asian pear is the most common phenomenon to the pear grower Particularly, when grown under subtropical to tropical climatic condition. Although one or two varieties of russet Asian pear cultivar...
Profitability of Yam-Maize-Soybean Enterprise among Resource Poor Farmers Using Herbicide for Weed Control in the Northern Guinea Savanna
This study examined the profitability of herbicide weed control in yam-maize-soybean enterprise, factors influencing it, as well as the problems encountered by the farmers using this weed control measures. The study was...
Effect of Dietary Replacement of Soyabean Meal with Toasted Sesame Seed on Performance, Tibia Bone Mineralisation and Gut Morphology of Broilers Chicken
Aims: This experiment was conducted to assess the effect of dietary replacement of soybean meal with toasted Sesame seed on performance, tibia bone mineralization and gut morphology of broiler chicken. Study Design: Th...
Efficacy of Selected Insecticide and Biopesticide Combinations against Major Insect Pests of Late Maturing Pigeonpea [Cajanus cajan (L.) Millsp.]
Aim: To evaluate the comparative efficacy of different chemical insecticide and biopesticide combinations against tur pod fly [Melanagromyza obtusa (Malloch)], gram pod borer [Helicoverpa armigera (Hübner)] and tur pod b...
Effect of Cow Dung, NPK and Their Combinations on Soil Properties and Performance of Sweet Potato (Ipomoea batatas L.) in Sudan Savanna, Nigeria
In Nigeria, one of the major problems confronting agriculture is poor soil fertility replenishment strategy that could allow sustainable production. Two field trials were conducted simultaneously in Sokoto (UDUS, Vegetab...