Pseudomonas aeruginosa and pyocyanin negatively act on the establishment of Enterobacteriaceae biofilm on a ceramic surface
Journal Title: International Journal of engineering Research and Applications - Year 2017, Vol 7, Issue 8
Abstract
The occurrence of biofilm on the porous surface of ceramic water filters and their candles may increase the risk of transmission of pathogens, especially enterobacteria. In addition, more than 90% of strains of Pseudomonas aeruginosa synthesize pyocyanin, a blue-green pigment, with antimicrobial activity against different organisms. The aim of this work was to verify the influence of P. aeruginosa and its pigment on the adhesion of four strains of Enterobacteriaceae to the surface of a ceramic coupon. Standard microbial suspensions (≈108CFU/mL) were transferred to reactors containing ceramic coupon with 100mm2 of area, immersed in sterile mineral water, to which had been added 0.5 g/L of yeast extract. Static incubation occurred over 240h at 37°C. Three conditions of contact were evaluated: at equal times, after 48h of advantage for P. aeruginosa and in presence of a subinhibitory concentration of pyocyanin. Axenic cultures were tested for comparison. The doubling-time and the densities of the enterobacteria in the aqueous medium and on the coupon, were determined. Pyocyanin exerted a disturbance on the adhesion, limiting the development of enterobacteria on the coupon, but did not reduce its population in the aqueous medium, suggesting that other species-specific factors participate in the phenomenon of antagonism.
Authors and Affiliations
Andrwey Augusto Galvão Viana, Rafael Xavier Martins, Gilanna Falcão Ferreira, Hermano Zenaide Neto, Ian Porto Gurgel do Amaral, Ulrich Vasconcelos
Keywords
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- EP ID EP391948
- DOI 10.9790/9622-0708022330.
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