Purification and Biochemical Characterization of L-Asparaginase from Aspergillus niger and Evaluation of Its Antineoplastic Activity
Journal Title: International Journal of Science and Research (IJSR) - Year 2015, Vol 4, Issue 2
Abstract
L-asparaginase is a chemotherapeutic drug used in the treatment of lymphoblastic leukemia. In the present study, the extracellular L-asparaginase produced by strain Aspergillus niger was purified, characterized. Moreover, its antiproliferative activity was evaluated. The apparent molecular weight of the enzyme was found to be 136 kDa. The optimal pH and temperature for the enzyme were 9.0°C and 40°C, respectively. The enzyme retained 100% of the activity at 40°C for 120 min. L-asparaginase against human normal cells did not show cytotoxicity. However, in the human leukemia cell line A431 the antiproliferative effects of L-asparaginase was observed after 96 h of incubation. For the first time, an L-asparaginase from fungus was evaluated as an antitumor agent in human cells lines and further investigations should be conducted to improve the knowledge about this enzyme.
A Review of Optimized Heat Treatment for Bearing Cup in Drive Shaft Assembly
Power transmission system has different constructive features according to the vehicle‘s driving type which can be front wheel drive, rear wheel drive or four wheel drive. In rear wheel drive system, elements of the syst...
Characterization of Black Shale of Chimiari Khyber Pakthunkhawa Region of Pakistan for Its Potential as Multi Minerals
The increasing demand has open the reach field to develop low grade ore deposits which were either technically impossible to develop or have serious environmental sequences or both. Black shale deposits are one of the lo...
The Upper Temperature Limit Above which the Influence of Dislocations Due to the Presence of the Reinforcement of the Composite 6061/SiC becomes Negligible
Studies that have already been made , shows that the acceleration of the precipitation kinetics of the semi coherent phase is attributed to the dislocations created during tempering, due to the difference in thermal expa...
Digitally Mediated Learning and Pre-Service Teachers in Less Resourced Regions
Digital technologies of today have challenged the traditional systems of education and have created potential new ways of delivery of knowledge and skills that require a critical look at learning and how it might be deli...
Pollution Study of Pallickal River water Based on Pesticidal Compounds
"Pallickal River is one of the smallest river of Kerala, it originate from the cliffs of ‘Nellimukal’ in Pathanamthitta. People are making use of this river water for their domestic use and agriculture. This river system...