PURIFICATION AND MOLECULAR WEIGHT DETERMINATION OF KERATINASE ISOLATED FROM STREPTOMYCES MALAYSIENSIS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2017, Vol 9, Issue 10
Abstract
Objective: The aim of the present study was to purify and determine the molecular weight of keratinase isolated from Streptomyces malaysiensis.Methods: For that purpose purification was done using ammonium sulphate and Sephadex-LH 100 column chromatography. Further, the fractions were pooled and subjected to molecular weight determination using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE).Results: The obtained results showed keratinase with 47.57% recovery, 3.5-fold purification and an estimated molecular mass of 27,000 Da. Keratinase showed an optimal activity at 60 οC and pH 8. Keratinase activity of the purified product was assayed with feather powder as a substrate. The isolated strain was identified as Streptomyces malaysiensis based on phylogenetic tree analysis. The strain isolated from termite mound soil showed the highest keratinase activity, which could be considered a microorganism of environmental origin.Conclusion: The production of keratinase on simple media with feathers as sole source allowing its production from the cheap substrate and a commercial production with low production cost. Stability in the presence of detergents, surfactants and solvents make this keratinase extremely useful for a biotechnological process involving keratin.
Authors and Affiliations
M. Pavani, G. Girijasankar, K. Mallika, G. Vidhya Sagar
INVESTIGATION OF THE SYNERGISTIC ANTIBACTERIAL ACTION OF COPPER NANOPARTICLES ON CERTAIN ANTIBIOTICS AGAINST HUMAN PATHOGENS
Objective: Resistance to antibacterial agents by pathogenic bacteria has emerged in recent years and is a major challenge for the healthcare industry. Copper nanoparticles (CuNPs) are known to be one of the multifunction...
APPLICATION OF QUALITY by DESIGN (CCD TECHNIQUE) FOR SIMULTANEOUS ESTIMATION OF CEFIXIME AND OFLOXACIN BY HPTLC METHOD
Objective: The present manuscript describes about simultaneous multiple response optimizations using the Derringer’s desirability function for the estimation of cefixime and ofloxacin in bulk and pharmaceutical dosage...
SOLID LIPID NANOPARTICLES AND NANOSTRUCTURED LIPID CARRIERS OF TOLNAFTATE: DESIGN, OPTIMIZATION AND IN-VITRO EVALUATION
Objective: The target of our work is the preparation of tolnaftate (TOL) loaded solid lipid nanoparticles (SLNs) as well as nanostructured lipid carriers (NLCs).Methods: The high shear homogenization method was chosen fo...
NOVEL GASTRO-RETENTIVE POLYMERIC MICROSPHERES: AN APPROACH FOR INCREASED BIOAVAILABILITY AND AN ONCE DAILY DOSING OF TERBUTALINE SULPHATE
Objective: The purpose of this study was to develop multi-unit alginate-copolymer adhesive microspheres to achieve a sustained release of terbutaline sulphate (TBS) and overcome the hepatic first pass effect so as to enh...
EVALUTION OF ANALGESIC ACTIVITY OF LEAF EXTRACTS OF PERGULARIA DAEMIA [FORSK] IN EXPERIMENTAL ANIMALS
Objective: Pergularia daemia [Forsk] has been used from long time in traditional medicine. The main objective of this work is to evaluate analgesic activity of leaf of Pergularia daemia [Forsk]Methods: Analgesic activity...