Purification of phenylalanine ammonia lyase (PAL) and peroxidase (POX) enzymes obtained from lucerne (Medicago sativa L. cv. Vertus) following inoculation with Verticillium albo-atrum
Journal Title: Journal of Research in Biology - Year 2012, Vol 2, Issue 4
Abstract
Phenylalanine ammonia lyase (EC 4.3.1.24, PAL) and peroxidase (EC 1.11.1.7, POX) enzyme activities were determined in seedlings of lucerne (Medicago sativa L. cv. Vertus) after the inoculation of V. albo-atrum (V2, a weak pathogenic isolate to lucerne). PAL and POX activities of lucerne inoculated with V2 were found significantly higher than that of the control group after 48 h inoculation. Partial purification of enzymes with 0-50% (NH4)2SO4 precipitation in control groups resulted in 2.74 and 2.42 fold increases. On the other hand, 1.78 and 1.74 fold increases in PAL and POX activities, respectively were evident in V2-inoculated plants. This study showed that the purification of the enzymes with little effort enabled us to see the differences between the treatments clearly. This type of work could be suggested where the protein concentration of the sample is low or a time-scale experiment is to be carried out to see the enzyme changes or to characterize the pathogen-related proteins.
Authors and Affiliations
Murat Dikilitas
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