Purification of phenylalanine ammonia lyase (PAL) and peroxidase (POX) enzymes obtained from lucerne (Medicago sativa L. cv. Vertus) following inoculation with Verticillium albo-atrum
Journal Title: Journal of Research in Biology - Year 2012, Vol 2, Issue 4
Abstract
Phenylalanine ammonia lyase (EC 4.3.1.24, PAL) and peroxidase (EC 1.11.1.7, POX) enzyme activities were determined in seedlings of lucerne (Medicago sativa L. cv. Vertus) after the inoculation of V. albo-atrum (V2, a weak pathogenic isolate to lucerne). PAL and POX activities of lucerne inoculated with V2 were found significantly higher than that of the control group after 48 h inoculation. Partial purification of enzymes with 0-50% (NH4)2SO4 precipitation in control groups resulted in 2.74 and 2.42 fold increases. On the other hand, 1.78 and 1.74 fold increases in PAL and POX activities, respectively were evident in V2-inoculated plants. This study showed that the purification of the enzymes with little effort enabled us to see the differences between the treatments clearly. This type of work could be suggested where the protein concentration of the sample is low or a time-scale experiment is to be carried out to see the enzyme changes or to characterize the pathogen-related proteins.
Authors and Affiliations
Murat Dikilitas
Keywords
Related Articles
- EP ID EP565616
- DOI -
- Views 55
- Downloads 0
Influence of various pH regimes on hatching success and hatching time of Clarias gariepinus (Butchell 1822) eggs
A laboratory experiment was conducted to assess the Hatching success and Hatching time of Clarias gariepinus eggs as influenced by various pH treatments. Experimental solution comprising various pH ranges (2-12) was cons...
Constraints in the control of animal trypanosomiasis by cattle farmers in coastal savannah of Ghana: Quality aspects of drug use
Cattle trypanosomiasis is a major constraint to livestock development in Ghana and is demonstrated by the fact that cattle farmers treat the disease by themselves. The main objective of this study is to identify the cons...
Effect of vitamins on digestive enzyme activities and growth performance of striped murrel Channa striatus
Five diets (diets incorporated with folic acid, vitamin B, vitamin C, vitamin/mineral mix and control) were used to determine their effects on survival, digestive enzyme activity, protein profiling and growth of striped...
Comparative efficacy of ivermectin (inj. vermic), fenbendazole (peraclear) and albendazole (almex-vet) against gastrointestinal nematodiasis in goats
This study aimed to evaluate the efficacy of ivermectin, fenbendazole and albendazole against gastrointestinal nematodes in naturally infected goats of government goat development farm, Sylhet, Bangladesh. The study incl...
Effect of Chromolaena odorata leaf extract on haematological profiles in Salmonellae typhi infested Wistar rats
Haematological indices provide an insight about the internal environment of a given organism. In this present study, the possible anti-haemototxic effect of Chromolaena odorata on Salmonellae typhi – induced haematotoxic...