Pyrrolidine dithiocarbamate alleviates the anti-tuberculosis drug-induced liver injury through JAK2/ STAT3 signaling pathway: An experimental study
Journal Title: Asian Pacific Journal of Tropical Medicine - Year 2017, Vol 10, Issue 5
Abstract
Objective: To study the effect of pyrrolidine dithiocarbamate (PDTC) on the antituberculosis drug-induced liver injury and the molecular mechanism. Methods: Clean male SD rats were selected as experimental animals and randomly divided into normal group, model group, PDTC group and AG490 group. Animal model of antituberculosis drug-induced liver injury was established by intragastric administration isoniazid + rifampicin. PDTC group received intraperitoneal injection of PDTC, and AG490 group received intraperitoneal injection of AG490. Twenty-eight days after intervention, the rats were executed, and the liver injury indexes, inflammation indexes and oxidative stress indexes in serum as well as JAK2/STAT3 expression, liver injury indexes, inflammation indexes and oxidative stress indexes in liver tissue were determined. Results: p-JAK2, p-STAT3, TNF-a, IL-1b, IL-6, ROS, 8-OHdG and MDA expression in liver tissue as well as TBIL, ALT, AST, g-GT, TNF-a, IL-1b, IL-6, 8-OHdG and MDA levels in serum of model group were significantly higher than those of normal group while p-JAK2, p-STAT3, TNF-a, IL-1b, IL-6, ROS, 8-OHdG and MDA expression in liver tissue as well as TBIL, ALT, AST, g-GT, TNF-a, IL-1b, IL-6, 8-OHdG and MDA levels in serum of PDTC group and AG490 group were significantly lower than those of model group. Conclusions: PDTC can inhibit the inflammation and oxidative stress mediated by JAK2/STAT3 signaling pathway to alleviate the anti-tuberculosis drug-induced liver injury.
Authors and Affiliations
Hong Zhang, Na Wei
Keywords
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- EP ID EP267425
- DOI 10.1016/j.apjtm.2017.05.010
- Views 97
- Downloads 0
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